PXD043487-1

PXD043487 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Proteins from honey bee larval hemolymph as key candidate nutrients for Varroa destructor artificial rearing
Description	Using artificial Parafilm™ dummies, we explored the nutrition of V. destructor females and assessed their survival when fed on hemolymph from bee larvae, pupae or adults. We compared the results to fully synthetic solutions and to filtered larval plasma. The results showed that the parasites could survive for several days or weeks on our different diets. Hemolymph from larva yielded the highest survival rates and filtered cell-free hemolymph was sufficient to maintain the mites for 14 days or more. This cell free solution thus theoretically contains all necessary nutrients for the mite survival. A proteomic analysis on the larval plasma outlined a list of 54 proteins among which several energy metabolism proteins such as vitellogenin, hexamerin or transferrin. These molecules represent key candidate nutrients that could be crucial for V. destructor survival.
HostingRepository	PRIDE
AnnounceDate	2023-10-24
AnnouncementXML	Submission_2023-10-24_14:56:54.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KarimArafah
SpeciesList	scientific name: Apis mellifera (Honeybee); NCBI TaxID: 7460;
ModificationList	S-pyridylethyl-L-cysteine; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-07-03 05:15:08	ID requested
⏵ 1	2023-10-24 14:56:55	announced
2	2023-11-14 09:12:39	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:13:14	announced	2024-10-22: Updated project metadata.

Publication List

Piou V, Vilarem C, Blanchard S, Strub JM, Bertile F, Bocquet M, Arafah K, Bulet P, V, é, tillard A, Artificial Rearing. Int J Mol Sci, 24(15):(2023) [pubmed]

Piou V, Vilarem C, Blanchard S, Strub JM, Bertile F, Bocquet M, Arafah K, Bulet P, V, é, tillard A, Artificial Rearing. Int J Mol Sci, 24(15):(2023) [pubmed]

Keyword List

submitter keyword: Mite
artificial dummy
hemolymph
parasite
protein
nutrition, insect physiology

submitter keyword: Mite

artificial dummy

hemolymph

parasite

protein

nutrition, insect physiology

Contact List

PhilippeBulet
contact affiliation	CR, University Grenoble Alpes, IAB Inserm 1209, CNRS UMR5309, 38000 Grenoble, France. Plateforme BioPark d'Archamps, 74160 Archamps, France.
contact email	philippe.bulet@biopark-archamps.org
lab head
KarimArafah
contact affiliation	Biopark Archamps
contact email	karim.arafah@biopark-archamps.org
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043487
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043487

PRIDE project URI

Repository Record List

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