PXD043413-2

PXD043413 is an original dataset announced via ProteomeXchange.

Title	Reduction of ribosomal expansion segments in yeast species of the Magnusiomyces/Saprochaete clade
Description	Ribosomes are ribonucleoprotein complexes highly conserved across all domains of life. The size differences of ribosomal RNAs (rRNAs) can be mainly attributed to variable regions termed expansion segments (ESs) protruding out from the ribosomal surface. Although the ESs were found to be involved in a range of processes including ribosome biogenesis and maturation, translation, and co-translational protein modification, their roles remain largely elusive. Here, we analyze the rRNAs of the yeasts from the Magnusiomyces/Saprochaete clade belonging to the basal lineages of the subphylum Saccharomycotina. We find that these yeasts lack more than 400 and 150 nt from the 25S and 18S rRNAs, respectively, when compared with “canonical” counterparts. With a few exceptions, the missing regions map to ESs thus representing a shift toward minimal rRNA structure raising a question about their roles. On the other hand, we show that the ribosomal protein inventories remained unaffected. We also show that the shortening of ESs is not limited to the species of the Magnusiomyces/Saprochaete clade as the rRNA reduction happened independently in several other lineages of the subphylum Saccharomycotina.
HostingRepository	PRIDE
AnnounceDate	2024-08-16
AnnouncementXML	Submission_2024-08-16_03:35:50.330.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peter Barath
SpeciesList	scientific name: Dipodascus albidus; NCBI TaxID: 27315; scientific name: Magnusiomyces capitatus; NCBI TaxID: 1095183;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-29 22:16:28	ID requested
1	2024-08-01 06:05:41	announced
⏵ 2	2024-08-16 03:35:51	announced	2024-08-16: Updated project metadata.
3	2024-10-22 06:52:06	announced	2024-10-22: Updated project metadata.

Br, á, zdovi, č F, Brejov, á B, Siv, á, kov, á B, Bar, á, th P, Ker, á, k F, Hodorov, á V, Vina, ř T, Tom, á, š, ka Ľ, Nosek J, Reduction of Ribosomal Expansion Segments in Yeast Species of the Magnusiomyces/Saprochaete Clade. Genome Biol Evol, 16(8):(2024) [pubmed]

10.1093/gbe/evae173;

submitter keyword: Magnusiomyces, yeast, ribosome,expansion segments, ribosomal RNA

Peter Barath
contact affiliation	Institute of Chemistry Slovak Academy of Sciences
contact email	peter.barath@savba.sk
lab head
Peter Barath
contact affiliation	Institute of Chemistry, Slovak Academy of Sciences
contact email	peter.barath@savba.sk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043413
     1. Label: PRIDE project
     2. Name: Reduction of ribosomal expansion segments in yeast species of the Magnusiomyces/Saprochaete clade