PXD043393 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Dietary Laminaria digitata and enzymatic supplementation on the ileum function on weaned piglets: a combined proteomics and metabolomics analysis |
Description | Laminaria digitata is a brown seaweed with prebiotic properties that has the potential to improve the response of weaned piglets to nutritional stress. However, its cell wall polysaccharides are not digested by the endogenous enzymes of monogastric animals. Alginate lyase has shown promise in degrading them under in vitro conditions. The objective of this study is to evaluate the effect of a 10% incorporation of L. digitata, and alginate lyase supplementation on the ileum proteome and metabolome, in a hypothesis generating approach. Control piglets increased the use of glucose as an enteric source of energy, demonstrated by the higher abundance of PKLR and PCK2 proteins and the lower concentration of glucose found in the tissue. Furthermore, seaweed inclusion promoted an increased abundance of proteins related with improved enterocyte structural integrity (ACTBL2, CRMP1, FLII, EML2 and MYLK), peptidase activity (NAALADL1, CAPNS1) and anti-inflammatory activity (C3), demonstrating improved intestinal function. Coherently, they lowered the abundance of apoptosis (ERN2) and proteolytic (DPP4) proteins. Alginate lyase supplementation seems to magnify the baseline effects of feeding the seaweed alone, by increasing the number of differential proteins in the same pathways, possibly as a consequence of increased intracellular nutrient release. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:57:21.947.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD043393 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Céline LECLERCQ |
SpeciesList | scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825; |
ModificationList | L-kynurenine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | TripleTOF 6600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-29 05:35:06 | ID requested | |
1 | 2023-09-29 07:05:00 | announced | |
⏵ 2 | 2023-11-14 08:57:22 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: proteomics, metabolomics,piglet, ileum, Laminaria digitata |
Contact List
Céline LECLERCQ |
contact affiliation | LIST-ERIN-BEAP Bascharage1 Luxembourg |
contact email | celine.leclercq@list.lu |
lab head | |
Céline LECLERCQ |
contact affiliation | LIST-ERIN-ERTP-BEAP-HAU platform |
contact email | celine.leclercq@list.lu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043393
- Label: PRIDE project
- Name: Dietary Laminaria digitata and enzymatic supplementation on the ileum function on weaned piglets: a combined proteomics and metabolomics analysis