PXD043371-2

PXD043371 is an original dataset announced via ProteomeXchange.

Title	Broadcasting ERK activation in the early Drosophila embryo
Description	The highly conserved ERK cascade controls development and physiology and is genetically deregulated in human birth defects, neurocognitive disorders, and cancers. While the list of the known ERK functions in different tissues and organisms is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here we show how new ERK functions can be systematically identified using targeted perturbations and global readouts of cell signaling systems. Our experimental model is the early Drosophila embryo, where ERK activation at the embryonic poles has thus far only been associated with transcriptional patterning of the terminal structures of the future larva. Based on the phosphoproteomic profiling after acute optogenetic perturbations of ERK signaling, we demonstrate that ERK activation at the poles is also critical for maintaining the speed and synchrony of embryonic cleavage cell cycles throughout the embryo. The presented approach sets the stage for a more complete understanding of signaling systems in health and disease.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:57:39.380.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Audrey Zhu
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 09:56:03	ID requested
1	2024-09-03 12:15:44	announced
⏵ 2	2024-10-22 06:57:39	announced	2024-10-22: Updated project metadata.

10.1016/J.DEVCEL.2024.08.004;

submitter keyword: ERK signaling,Phosphoproteomics, optogenetics, cell cycle

Martin Helmut Wuhr
contact affiliation	Wuhr Lab, Molecular Biology and the Lewis-Sigler Institute for Integrative Genomics, Princeton University, USA
contact email	wuhr@princeton.edu
lab head
Audrey Zhu
contact affiliation	Princeton University
contact email	awzhu@princeton.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD043371

PRIDE project URI

• PRIDE
  1. PXD043371
     1. Label: PRIDE project
     2. Name: Broadcasting ERK activation in the early Drosophila embryo