PXD043368-1

PXD043368 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Involvement of the AKT pathway in resistance to erlotinib and cabozantinib in triple negative breast cancer cell lines.
Description	Resistance to tyrosine kinase inhibitors (TKIs) presents a growing challenge in the development of therapeutic targets for cancers such as triple negative breast cancer (TNBC), where conventional therapies are ineffective for combatting systemic disease. Due to increased expression, the receptor tyrosine kinases EGFR (epidermal growth factor receptor) and c-Met are potential targets for treatment. However, targeted anti-EGFR and anti-c-Met therapies have faced mixed results in clinical trials due to acquired resistance. We hypothesize that the upregulation of kinases within the EGFR and c-Met signaling axes contribute to the development of acquired erlotinib and cabozantinib resistance. To test this, we developed two separate models for cabozantinib and erlotinib resistance using the MDA-MB-231 and MDA-MB-468 cell lines, respectively. With a SILAC (Stable Isotope Labeling of Amino acids in Cell Cul-ture)-labeled quantitative mass spectrometry proteomics approach, we assessed the effects of erlotinib- or cabozantinib resistance on the phosphoproteome, proteome and kinome. Using this integrated proteomics approach, we identified several potential kinase mediators of cabozan-tinib-resistance and confirmed the contribution of AKT1 to erlotinib-resistance in TNBC resistant cell lines.
HostingRepository	jPOST
AnnounceDate	2024-06-28
AnnouncementXML	Submission_2024-06-27_08:00:04.388.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Cory Lefebvre
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	S-carboxamidomethyl-L-cysteine; 6x(13)C labeled L-lysine; 6x(13)C labeled L-arginine; Label:13C(6)15N(2); Label:13C(6)15N(4); L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine
Instrument	LTQ Orbitrap Velos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-28 08:03:15	ID requested
⏵ 1	2024-06-27 08:00:04	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: SILAC, proteomics, phosphoproteomics, triple negative breast cancer, erlotinib, cabozantinib

submitter keyword: SILAC, proteomics, phosphoproteomics, triple negative breast cancer, erlotinib, cabozantinib

Contact List

Dr. Alison Allan
lab head
Cory Lefebvre
contact affiliation	University of Western Ontario
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002219/