PXD043352-2

PXD043352 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Global quantitative proteomics of mouse hippocampus during ageing
Description	Understanding the molecular changes associated with the aged brain forms the basis for developing potential strategies for slowing cognitive decline associated with normal aging. Focusing on the hippocampus, a critical brain region involved in learning and memory, we employed tandem mass tag methodology to investigate proteomic changes that occur in advanced aged (20-month) relative to young (3-month) C57BL/6 mice. Our analysis revealed a total of 324 proteins that were significantly altered in the old mice relative to the young mice. Upregulated proteins (236 total proteins) were enriched within several age-related processes, such as the adaptive immune response and molecular metabolic pathways, whereas downregulated proteins (88 total proteins) were mainly involved in axonogenesis and growth cone-related processes. By categorising proteins according to their cell-type enrichment in the brain, a general upregulation was observed in the level of proteins preferentially expressed in microglia, astrocytes, and oligodendrocytes. In contrast, proteins with neuron-specific expression display an overall age-related downregulation. By integrating our proteomic and transcriptomic data, we discovered a mild but significant positive correlation between mRNA and protein expression changes in the aged hippocampus. Therefore, this proteomic data is a valuable resource for age-related molecular studies.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:18:11.307.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043352
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Victor Anggono
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-27 16:36:37	ID requested
1	2023-12-20 02:11:35	announced
⏵ 2	2024-10-22 06:18:11	announced	2024-10-22: Updated project metadata.

Publication List

10.6019/PXD043352;
10.1002/PMIC.202300276;

10.6019/PXD043352;

10.1002/PMIC.202300276;

Keyword List

submitter keyword: proteomics,hippocampus, mass spectrometry, cognitive decline, aging

submitter keyword: proteomics,hippocampus, mass spectrometry, cognitive decline, aging

Contact List

Victor Anggono
contact affiliation	The University of Queensland
contact email	v.anggono@uq.edu.au
lab head
Victor Anggono
contact affiliation	The University of Queensland
contact email	v.anggono@uq.edu.au
dataset submitter

Full Dataset Link List

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PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/12/PXD043352

PRIDE project URI

Repository Record List

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