PXD043339-1

PXD043339 is an original dataset announced via ProteomeXchange.

Title	Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds
Description	The bark represents the outer protective layer of trees. It contains high concentrations of antimicrobial extractives, in addition to regular wood polymers. It represents a huge underutilized side stream in forestry, but biotechnological valorization is hampered by a lack of knowledge on microbial bark degradation. Many fungi are efficient lignocellulose degraders, and here, spruce bark degradation by five species, Dichomitus squalens, Rhodonia placenta, Penicillium crustosum, Trichoderma sp. B1, and Trichoderma reesei, was mapped, by continuously analyzing chemical changes in the bark over six months. The study reveals how fungi from different phyla degrade bark using diverse strategies, regarding both wood polymers and extractives, where toxic resin acids were degraded by Basidiomycetes but unmodified/tolerated by Ascomycetes. Proteome analyses of the white-rot D. squalens revealed several proteins, with both known and unknown functions, that were specifically upregulated during growth on bark. This knowledge can accelerate improved utilization of an abundant renewable resource.
HostingRepository	PRIDE
AnnounceDate	2024-05-04
AnnouncementXML	Submission_2024-05-04_03:09:40.207.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Amanda Ristinmaa
SpeciesList	scientific name: Dichomitus squalens; NCBI TaxID: 114155;
ModificationList	acetylated residue; 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-27 05:02:49	ID requested
⏵ 1	2024-05-04 03:09:40	announced
2	2024-10-22 06:38:08	announced	2024-10-22: Updated project metadata.

10.1016/J.BIORTECH.2024.130768;

submitter keyword: CAZymes, proteomics,Spruce bark, polysaccharides, fungi, white-rot

Johan Larsbrink
contact affiliation	Chalmers University of Technology
contact email	johan.larsbrink@chalmers.se
lab head
Amanda Ristinmaa
contact affiliation	Chalmers University of Technology
contact email	amandar@chalmers.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043339
     1. Label: PRIDE project
     2. Name: Analyses of long-term fungal degradation of spruce bark reveals varying potential for catabolism of polysaccharides and extractive compounds