PXD043322-1

PXD043322 is an original dataset announced via ProteomeXchange.

Title	Systematic discovery of linear ubiquitination mediated neo-protein-protein interactions across mouse tissues
Description	HOIP truncations proteins GST-PUB, GST-NZF, GST-UBA and GST-RBR-LDD were purified, GST was used as a negative control. The tissue lysates from the C57BL/6 mice were incubated with glutathione-S-transferase (GST) beads which bound to the GST-HOIP truncation proteins beforehand. The pull-down component were separated by SDS-PAGE and stained with Coomassie brilliant blue. The band were cut off and subject to mass spectrometry.
HostingRepository	iProX
AnnounceDate	2023-06-26
AnnouncementXML	Submission_2024-02-28_02:44:41.784.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lei Li
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 19:27:41	ID requested
⏵ 1	2024-02-28 02:44:42	announced
2	2025-05-21 18:59:56	announced	2025-05-22: Add Publication.

Dataset with its publication pending

submitter keyword: Quantitative HOIP interactome profiles, mouse tissues

Lingqiang Zhang
contact affiliation	State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics
contact email	zhanglq@nic.bmi.ac.cn
lab head
Lei Li
contact affiliation	National Center for Protein Sciences (Beijing)
contact email	lilei_birm@163.com
dataset submitter

iProX dataset URI