PXD043317-1

PXD043317 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Syphilis and the Host; multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis.
Description	Whole cell lysates from hCMEC/d3 brain microvascular endothelial cells grown in medium and heavy SILAC media and exposed to Treponema pallidum or control media for 5 hours. Prior to Mass-spec analysis, each sample was fractionated into 12 fractions via high-pH reversed-phase liquid chromatography, then each fraction was analyzed by liquid chromatography tandem mass spectrometry. Exposures were repeated with 4 biological replicates, which we define as individual sample wells. In samples 1&2 medium SILAC cells were exposed to Treponema pallidum, and heavy SILAC cells were exposed to control media. In Samples 3&4 medium SILAC cells were exposed to control media, and heavy SILAC cells were exposed to Treponema pallidum. Cells were grown in D4-lysine (37.5 mg/liter) and 13C6-arginine (21.75 mg/liter) for medium isotope-labeled cells, or 13C615N2-lysine (38.5 mg/liter) and 13C615N4-arginine (22.25 mg/liter) for heavy isotope-labeled cells.
HostingRepository	MassIVE
AnnounceDate	2023-09-13
AnnouncementXML	Submission_2023-09-13_14:58:10.395.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sean
SpeciesList	scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationList	unknown modification
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-26 16:37:33	ID requested
⏵ 1	2023-09-13 14:58:10	announced

Publication List

Waugh S, Ranasinghe A, Gomez A, Houston S, Lithgow KV, Eshghi A, Fleetwood J, Conway K, Reynolds LA and Cameron CE. Syphilis and the host
multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis. Front. Microbiol. 14:1254342. doi: 10.3389/fmicb.2023.1254342.

Waugh S, Ranasinghe A, Gomez A, Houston S, Lithgow KV, Eshghi A, Fleetwood J, Conway K, Reynolds LA and Cameron CE. Syphilis and the host

multi-omic analysis of host cellular responses to Treponema pallidum provides novel insight into syphilis pathogenesis. Front. Microbiol. 14:1254342. doi: 10.3389/fmicb.2023.1254342.

Keyword List

submitter keyword: syphilis, endothelial, Treponema pallidum

submitter keyword: syphilis, endothelial, Treponema pallidum

Contact List

Dr. Caroline Cameron
contact affiliation	University of Victoria
contact email	caroc@uvic.ca
lab head
Sean
contact affiliation	University of victoria
contact email	swaugh@uvic.ca
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092275/