PXD043244 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic and phosphoproteomic profiling of mouse embryonic fibroblasts |
| Description | Fibroblasts are the most common cell type in stroma and function in the support and repair of most tissues. Mouse embryonic fibroblasts (MEFs) are amenable to isolation and rapid growth in culture. To facilitate their use as a research tool, we have performed a comprehensive proteomic and phosphoproteomic characterisation of wildtype primary MEFs from C57BL/6 mice. EIF2/4 and MTOR signalling pathways were enriched in both the proteome and phosphoproteome, along with extracellular matrix (ECM) and cytoskeleton associated pathways. Consistent with this, kinase enrichment analysis identified activation of P38A, P90RSK, P70RSK, and MTOR. Cell surface markers and matrisome proteins were also annotated. |
| HostingRepository | PRIDE |
| AnnounceDate | 2023-11-14 |
| AnnouncementXML | Submission_2023-11-14_09:06:08.081.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD043244 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Nicole Verrills |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
| ModificationList | phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-06-23 08:14:07 | ID requested | |
| 1 | 2023-10-20 04:39:22 | announced | |
| ⏵ 2 | 2023-11-14 09:06:12 | announced | 2023-11-14: Updated project metadata. |
| 3 | 2024-10-22 06:06:39 | announced | 2024-10-22: Updated project metadata. |
Publication List
| 10.6019/PXD043244; |
| Chen Y, Roselli S, Panicker N, Brzozowski JS, Skerrett-Byrne DA, Murray HC, Verrills NM, Proteomic and phosphoproteomic characterisation of primary mouse embryonic fibroblasts. Proteomics, 24(7):e2300267(2024) [pubmed] |
Keyword List
| submitter keyword: Phosphoproteomics, Proteomics, fibroblasts |
Contact List
| Nicole Verrills |
|---|
| contact affiliation | School of Biomedical Sciences and Pharmacy, College of Health, Medicine and Wellbeing, University of Newcastle, Callaghan, and Precision Medicine Program, Hunter Medical Research Institute, NSW, 2308, Australia |
| contact email | nikki.verrills@newcastle.edu.au |
| lab head | |
| Nicole Verrills |
|---|
| contact affiliation | School of Biomedical Sciences and Pharmacy Faculty of Health and Medicine, University of Newcastle, and Cancer Research Program, Hunter Medical Research Institute, Callaghan, NSW, 2308, Australia |
| contact email | Nikki.Verrills@newcastle.edu.au |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043244 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD043244
- Label: PRIDE project
- Name: Proteomic and phosphoproteomic profiling of mouse embryonic fibroblasts
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