PXD043201-3

PXD043201 is an original dataset announced via ProteomeXchange.

Title	Endogenous retroviruses promote prion-like spreading of proteopathic seeds
Description	Prion-like spreading of protein misfolding is characteristic for neurodegenerative diseases, but the exact mechanisms of intercellular protein aggregate dissemination remain unresolved. Evidence accumulates that endogenous retroviruses, remnants of viral germline infections that are normally epigenetically silenced, become upregulated in neurodegenerative diseases such as amyotrophic lateral sclerosis and tauopathies. Here we uncover that activation of endogenous retroviruses affects prion-like spreading of proteopathic seeds. To identify changes in the proteome of donor cells that might contribute to protein aggregate spreading, we performed mass spectrometry analyses of total cell lysates and donor EV fractions using N2a cells expressing HA epitope-tagged Sup35 NM prion protein at early (P07) and late passages (P16) post cryopreservation. Among the proteins increased in donor cells and EVs upon prolonged culture, we identified mouse endogenous MLV retrovirus proteins to be highly increased.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:06:15.147.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stephan Mueller
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-22 03:02:57	ID requested
1	2023-10-18 08:21:37	announced
2	2023-11-14 09:06:10	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 06:06:16	announced	2024-10-22: Updated project metadata.

10.1038/s41467-023-40632-z;

Liu S, Heum, ü, ller SE, Hossinger A, M, ü, ller SA, Buravlova O, Lichtenthaler SF, Denner P, Vorberg IM, Reactivated endogenous retroviruses promote protein aggregate spreading. Nat Commun, 14(1):5034(2023) [pubmed]

submitter keyword: N2a,Prion, Retrovirus

Dr Stefan F. Lichtenthaler
contact affiliation	DZNE Munich, Neuroproteomics, Feodor-Lynen Str. 17, 81377 Munich, Germany
contact email	stefan.lichtenthaler@dzne.de
lab head
Stephan Mueller
contact affiliation	DZNE Munich Neuroproteomics
contact email	stephan.mueller@dzne.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043201

PRIDE project URI

• PRIDE
  1. PXD043201
     1. Label: PRIDE project
     2. Name: Endogenous retroviruses promote prion-like spreading of proteopathic seeds