PXD043162-1

PXD043162 is an original dataset announced via ProteomeXchange.

Title	Proteomic profiling for elucidating the molecular mechanism in early seed development in rice
Description	Understanding the molecular mechanisms underlying early seed development is important for improving grain yield and quality in crop plants. To investigate the molecular mechanisms that occur during early seed development, we performed comparative label-free quantitative proteomic analysis on developing WT rice seeds between 0 and 1 day after pollination (DAP). A total of 5231 proteins were identified, and 902 proteins showed differential accumulation between 0 and 1 DAP seeds. Further analysis focused on the proteins preferentially expressed at 1 DAP and revealed an enrichment of proteins involved in DNA replication and pyrimidine biosynthetic pathways. Notably, the transcript of OsCTPS1, a cytidine triphosphate synthase known to play an essential role in early endosperm development, did not differ between 0 and 1 DAP, but OsCTPS1 protein accumulated more specifically at 1 DAP than at 0 DAP. We then found that inhibiting phosphorylation increased the stability of this protein. Furthermore, in osctps1-2, minichromosome maintenance (MCM) proteins were significantly reduced compared to WT at 1 DAP, and mutations in OsMCM5 caused defects in seed development. In this study, we tried to understand the molecular mechanisms of early seed development at the post-transcriptional regulation.
HostingRepository	PRIDE
AnnounceDate	2023-12-05
AnnouncementXML	Submission_2023-12-05_01:00:49.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sun Tae Kim
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-21 01:54:51	ID requested
⏵ 1	2023-12-05 01:00:49	announced
2	2024-10-22 06:16:48	announced	2024-10-22: Updated project metadata.

Yoon J, Min CW, Kim J, Baek G, Kim D, Jang JW, Gupta R, Kim ST, Cho LH, Quantitative Proteomic Analysis Deciphers the Molecular Mechanism for Endosperm Nuclear Division in Early Rice Seed Development. Plants (Basel), 12(21):(2023) [pubmed]

10.3390/plants12213715;

submitter keyword: Rice, Early seed development, CTP synthase,Proteomic profiling, MCM proteins, Protein stability

Sun Tae Kim
contact affiliation	Department of Plant Biosience, Pusan National University, Miryang 50463, Republic of Korea
contact email	stkim5505@gmail.com
lab head
Sun Tae Kim
contact affiliation	Pusan National University
contact email	stkim5505@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043162
     1. Label: PRIDE project
     2. Name: Proteomic profiling for elucidating the molecular mechanism in early seed development in rice