PXD043141-1

PXD043141 is an original dataset announced via ProteomeXchange.

Title	Quantitative proteomic analysis and verification identify global protein profiling dynamics in pig saliva during the estrus cycle
Description	The current estrus detection method is generally time-consuming and has low accuracy. As such, a deeper understanding of the physiological processes during the estrus cycle accelerates the development of estrus detection efficiency and accuracy. This study used label-free acquisition mass spectrometry to explore salivary proteome profiles during the estrus cycle (day −3, day 0, day 3, and day 8) in pigs. A total of 1155 proteins were identified in the label-free analysis, of which 115 were identified as differentially expressed proteins (DEPs) among different groups (P ≤ 0.05). Functional annotation revealed that the DEPs were clustered in calcium ion binding, actin cytoskeleton, and lyase activity. Parallel reaction monitoring (PRM) was applied to verify the relative profiles of protein expression, in which PHB domain-containing protein, growth factor receptor-bound protein 2, elongation factor Tu, carboxypeptidase D, carbonic anhydrase, and trefoil factor 3 were confirmed to be consistent in both label-free and PRM approaches. Comparative proteomic assays on saliva would increase our knowledge of the estrus cycle in sows and provide potential methods for estrus detection.
HostingRepository	PRIDE
AnnounceDate	2023-09-15
AnnouncementXML	Submission_2023-09-15_01:25:40.699.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HaiyunXin
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-20 08:29:50	ID requested
⏵ 1	2023-09-15 01:25:41	announced

Dataset with its publication pending

submitter keyword: sow

estrus detection

saliva

quantitative proteomics

differentially expressed proteins

reproduction efficiency;

JianhaoLi
contact affiliation	State Key Laboratory of Swine and Poultry Breeding Industry/Guangdong Key Laboratory of Animal Breeding and Nutrition/Institute of Animal Science/Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China;
contact email	3562047763@qq.com
lab head
HaiyunXin
contact affiliation	The State Key Laboratory of Livestock and Poultry Breeding/Guangdong Key Laboratory of Animal Breeding and Nutrition/Institute of Animal Science/Guangdong Academy of Agricultural Sciences
contact email	523727022@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/09/PXD043141

PRIDE project URI

• PRIDE
  1. PXD043141
     1. Label: PRIDE project
     2. Name: Quantitative proteomic analysis and verification identify global protein profiling dynamics in pig saliva during the estrus cycle