PXD043140-1

PXD043140 is an original dataset announced via ProteomeXchange.

Title	Thylakoid protein turnover rate measurement in Col-0, stn7-1 and tap38-1 by 15N labelling
Description	State transitions involve the phosphorylation and dephosphorylation of light-harvesting complex II, catalyzed by the thylakoid kinase STN7 and the phosphatase TAP38/PPH1 in algae and higher plants. These transitions enable algae and plants to coordinate the activation of photosystem I and II. However, the impact of state transitions on the turnover of thylakoid proteins remains unclear. In this study, we systematically measured the in vivo turnover rates of thylakoid proteins in stn7-1 and tap38-1 knockout mutants, which are locked in permanent State I and State II, respectively, and compared these rates to those in wild-type Arabidopsis. Notably, photosynthetic proteins such as D1, LHCII, and PSAB exhibited opposing turnover rates in the two mutant lines, suggesting that State I and State II regulate their turnover antagonistically. Strikingly, these changes in turnover did not correspond to changes in protein abundance but correlate with distribution of activation energy and intensity of electron flows, highlighting the role of activation energy and electron flow in protein turnover and homeostasis maintenance. Overall, this study elucidates a novel role of state transitions in regulation of the turnover of photosynthetic electron transport chain protein complexes, providing new insights into the reason for existence of state transition in plants, most likely through regulating energy distribution associated electron flows.
HostingRepository	PRIDE
AnnounceDate	2025-01-23
AnnouncementXML	Submission_2025-01-23_06:30:35.925.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuqi Hou
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 240

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-20 07:22:38	ID requested
⏵ 1	2025-01-23 06:30:36	announced

Dataset with its publication pending

submitter keyword: phosphorylation, photosynthetic complexes,stage transition

Lei Li
contact affiliation	Nankai University,Lei Li Lab,college of Life Sciences,Tianjin China
contact email	lei.li@nankai.edu.cn
lab head
Yuqi Hou
contact affiliation	Nankai University,leili lab
contact email	1120200476@mail.nankai.edu.cn
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043140
     1. Label: PRIDE project
     2. Name: Thylakoid protein turnover rate measurement in Col-0, stn7-1 and tap38-1 by 15N labelling