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PXD043135-2

PXD043135 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSTPP-UP: A alternative method for drug target identification using protein thermal stability
DescriptionThermal proteome profiling (TPP) has significantly advanced the field of drug discovery by facilitating proteome-wide identification of drug targets and off-targets. However, TPP has not been widely applied for high-throughput drug screenings, since the method is labor intensive and requires a lot of measurement time on a mass spectrometer. Here, we present Single-tube TPP with Uniform Progression (STPP-UP), which significantly reduces both the amount of required input material and measurement time, while retaining the ability to identify drug targets for compounds of interest.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:25:38.553.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterDick Zijlmans
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480; Q Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-20 03:55:11ID requested
12023-10-24 05:43:58announced
22023-11-14 08:25:38announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: protein stability, drug discovery, thermal proteomics,STPP-UP
Contact List
Michiel Vermeulen
contact affiliationDepartment of Molecular Biology, Faculty of Science, Radboud Institute for Molecular Life Sciences, Oncode Institute, Radboud University Nijmegen, Nijmegen, The Netherlands. Division of Molecular Genetics, The Netherlands Cancer Institute, Amsterdam, The Netherlands.
contact emailmichiel.vermeulen@ru.nl
lab head
Dick Zijlmans
contact affiliationRadboud Universiteit Nijmegen
contact emaild.zijlmans@science.ru.nl
dataset submitter
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Dataset FTP location
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PRIDE project URI
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