PXD043123-2

PXD043123 is an original dataset announced via ProteomeXchange.

Title	Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition
Description	Phenolic acids (PAs) secreted by donor plants suppress the growth of their susceptible plant neighbours. However, how structurally diverse ensembles of PAs are perceived by plants to mediate interspecific competition remains a mystery. Here, we show that a plant stress granule (SG) marker, RNA-BINDING PROTEIN 47B (RBP47B), is a sensor of PAs in Arabidopsis. PAs including salicylic acid (SA), 4-hydroxybenzoic acid, protocatechuic acid, etc. directly bind RBP47B, promote its phase separation and trigger SG formation accompanied by global translation inhibition. SA-induced global translation inhibition depends on RBP47 family members. RBP47s regulate the proteome rather than the absolute quantity of SG. The rbp47 quadruple mutant shows a reduced sensitivity to the inhibitory effect of the PA mixture as well as to that of PA-rich rice when tested in a co-culturing ecosystem. Our study discovers RBP47B as the long sought-after PA sensor and unveils PA-induced SG formation and translation inhibition as one of the PA perception mechanisms.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:47:28.332.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhouli Xie
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 23:28:45	ID requested
1	2023-08-28 17:56:21	announced
⏵ 2	2023-11-14 07:47:28	announced	2023-11-14: Updated project metadata.
3	2024-10-22 06:02:01	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Salicylic acid (SA),Phenolic acid, Stress granules (SG), RNA-BINDING PROTEIN 47B (RBP47B)

Wei Wang
contact affiliation	State Key Laboratory for Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
contact email	oneway1985@pku.edu.cn
lab head
Zhouli Xie
contact affiliation	Peking University
contact email	zhou_lixie@126.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD043123

PRIDE project URI

• PRIDE
  1. PXD043123
     1. Label: PRIDE project
     2. Name: Phenolic acid-induced phase separation and translation inhibition mediate plant interspecific competition