PXD043103-1

PXD043103 is an original dataset announced via ProteomeXchange.

Title	Immunorecognition of Streptococcus mutans secreted proteins protects against caries by limiting tooth adhesion
Description	Childhood caries is an extremely common childhood chronic disease, affecting 60–90% of children in industrialized countries. It results in lesions in both the primary and permanent dentitions, hospitalizations and emergency room visits, high treatment costs, loss of school days, diminished ability to learn increases the risk of caries in adulthood. Streptococcus mutans is a key bacteria in caries development. While multiple caries risk factors have been identified, significant interpersonal variability not explained by known risk factors still exists. The immune system generates a personal antibody repertoire that helps maintain a balanced and healthy oral microbiome. Using mass-spectrometry, we probed in an hypothesis-free manner which S. mutans proteins are identified by antibodies of children with low and high DMFT (decayed, missing, filled teeth) scores. We identified a core set of proteins, recognized by the immune system of most individuals. This set was enriched with proteins enabling bacterial adhesion, and included glucosyltransferases and glucan-binding proteins known to be important for S. mutans cariogenicity. To explore the physiological relevance of these findings, we tested the ability of saliva from caries free individuals in preventing S. mutans from binding to the tooth surface. Indeed, saliva from individuals with caries free prevented S. mutans binding to teeth. These findings map the S. mutans proteome targeted by the immune system and suggest that inhibiting tooth attachment is a primary mechanism used by the immune system to maintain oral balance and prevent caries. These findings provide new insights into the role of the immune system in maintaining oral health and preventing caries development.
HostingRepository	PRIDE
AnnounceDate	2023-06-26
AnnouncementXML	Submission_2023-06-26_07:35:46.060.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DanielBar
SpeciesList	scientific name: Streptococcus mutans UA159; NCBI TaxID: 210007;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 08:36:13	ID requested
⏵ 1	2023-06-26 07:35:46	announced
2	2024-10-22 05:50:51	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: caries

IgA

mass-spectrometry

immunorecognition

Daniel ZviBar
contact affiliation	Faculty of Medicine, Tel Aviv University, Israel
contact email	dbar@tauex.tau.ac.il
lab head
DanielBar
contact affiliation	Tel Aviv University
contact email	dbar@tauex.tau.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD043103

PRIDE project URI

• PRIDE
  1. PXD043103
     1. Label: PRIDE project
     2. Name: Immunorecognition of Streptococcus mutans secreted proteins protects against caries by limiting tooth adhesion