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PXD043088-2

PXD043088 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of microbe-containing phagosomes using APEX2-based proximity labeling
DescriptionPhagocytosis is the process by which myeloid phagocytes bind and internalize potentially dangerous microbes. During phagocytosis, innate immune receptors and the associated signaling adapters are localized to the maturing phagosome compartment. We used proximity labeling of phagosomal contents (PhagoPL) to identify proteins localizing to phagosomes containing model yeast and bacteria and identified programmed death-ligand 1 (PD-L1) as a protein that specifically enriches in phagosomes containing yeast. We found that PD-L1 directly binds to yeast upon processing in phagosomes and influences production of a subset of inflammatory cytokines and chemokines produced when macrophages encounter yeast.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:36:58.387.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterWei Yang
SpeciesList scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationListacetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-19 01:54:00ID requested
12024-04-22 14:25:11announced
22024-10-22 06:36:58announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: phagocytosis, APEX2, PD-L1,Proximity labeling
Contact List
Wei Yang
contact affiliationCedars-Sinai Medical Center Los Angeles, CA 90048
contact emailomician@gmail.com
lab head
Wei Yang
contact affiliationStony Brook University
contact emailwei.yang@cshs.org
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
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