PXD043088-2

PXD043088 is an original dataset announced via ProteomeXchange.

Title	Characterization of microbe-containing phagosomes using APEX2-based proximity labeling
Description	Phagocytosis is the process by which myeloid phagocytes bind and internalize potentially dangerous microbes. During phagocytosis, innate immune receptors and the associated signaling adapters are localized to the maturing phagosome compartment. We used proximity labeling of phagosomal contents (PhagoPL) to identify proteins localizing to phagosomes containing model yeast and bacteria and identified programmed death-ligand 1 (PD-L1) as a protein that specifically enriches in phagosomes containing yeast. We found that PD-L1 directly binds to yeast upon processing in phagosomes and influences production of a subset of inflammatory cytokines and chemokines produced when macrophages encounter yeast.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:36:58.387.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Yang
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-19 01:54:00	ID requested
1	2024-04-22 14:25:11	announced
⏵ 2	2024-10-22 06:36:58	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: phagocytosis, APEX2, PD-L1,Proximity labeling

Wei Yang
contact affiliation	Cedars-Sinai Medical Center Los Angeles, CA 90048
contact email	omician@gmail.com
lab head
Wei Yang
contact affiliation	Stony Brook University
contact email	wei.yang@cshs.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD043088

PRIDE project URI

• PRIDE
  1. PXD043088
     1. Label: PRIDE project
     2. Name: Characterization of microbe-containing phagosomes using APEX2-based proximity labeling