PXD043084-1

PXD043084 is an original dataset announced via ProteomeXchange.

Title	ZNF524 directly interacts with telomeric DNA and supports telomere integrity
Description	Telomeres are nucleoprotein structures at the ends of linear chromosomes. In humans, they consist of TTAGGG repeats, which are bound by dedicated proteins such as the shelterin complex. This complex blocks unwanted DNA damage repair at telomeres, e.g. by suppressing non-homologous end joining (NHEJ) through its subunit TRF2. We here describe ZNF524, a zinc finger protein that directly binds telomeric repeats with nanomolar affinity and reveal the base-specific sequence recognition by co-crystallization with telomeric DNA. ZNF524 localizes to telomeres and specifically maintains the presence of the TRF2/RAP1 subcomplex at telomeres without affecting other shelterin members. Loss of ZNF524 concomitantly results in an increase in DNA damage signaling and recombination events. Overall, ZNF524 is a direct telomere-binding protein involved in the maintenance of telomere integrity.
HostingRepository	PRIDE
AnnounceDate	2023-10-21
AnnouncementXML	Submission_2023-10-21_03:03:47.374.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	DennisKappei
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-18 21:36:12	ID requested
⏵ 1	2023-10-21 03:03:47	announced
2	2024-10-22 06:06:49	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: TRF2, genome integrity, ALT, Telomeres, RAP1,ZNF524, osteosarcoma

DennisKappei
contact affiliation	Cancer Science Institute of Singapore (CSI), National University of Singapore (NUS
contact email	dennis.kappei@nus.edu.sg
lab head
DennisKappei
contact affiliation	Cancer Science Institute of Singapore
contact email	dennis.kappei@nus.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD043084

PRIDE project URI

• PRIDE
  1. PXD043084
     1. Label: PRIDE project
     2. Name: ZNF524 directly interacts with telomeric DNA and supports telomere integrity