PXD043076-1

PXD043076 is an original dataset announced via ProteomeXchange.

Title	A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells
Description	The devastating blast fungus Magnaporthe oryzae elaborates invasive hyphae (IH) in living rice cells during early infection, separated from host cytoplasm by plant-derived interfacial membranes, but the metabolic strategies underpinning this fundamental intracellular biotrophic growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with multiomic approaches, we show how the M. oryzae serine/threonine protein kinase Rim15 coordinates cycles of autophagy and glutaminolysis in IH – the latter through phosphorylation of NAD-dependent glutamate dehydrogenase – to reactivate TOR and promote biotrophic growth. Deleting RIM15 attenuated IH growth and triggered plant immunity; these defects were fully remediated by exogenous α-ketoglutarate treatment, but glucose treatment only suppressed host defenses. Our results together suggest that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression.
HostingRepository	PRIDE
AnnounceDate	2023-07-10
AnnouncementXML	Submission_2023-07-10_12:31:04.341.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	GangLi
SpeciesList	scientific name: Magnaporthe sp. MG08; NCBI TaxID: 1758543;
ModificationList	phosphorylated residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-18 08:48:25	ID requested
⏵ 1	2023-07-10 12:31:04	announced

Dataset with its publication pending

submitter keyword: glutaminolysis, autophagy, α-ketoglutarate, antioxidation, TOR signaling, biotrophy, Rim15,Rice blast disease, Magnaporthe oryzae, biotrophic interface, plant defense suppression

Richard A.Wilson
contact affiliation	Department of Plant Pathology, University of Nebraska, Lincoln,NE
contact email	rwilson10@unl.edu
lab head
GangLi
contact affiliation	University of Nebraska-Lincoln
contact email	lig.genetics@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043076
     1. Label: PRIDE project
     2. Name: A protein kinase coordinates cycles of autophagy and glutaminolysis in invasive hyphae of the fungus Magnaporthe oryzae within rice cells