PXD043058-2

PXD043058 is an original dataset announced via ProteomeXchange.

Title	Extracellular Vesicles in COVID-19 convalescence can regulate T cell metabolism and function
Description	Long-term T cell dysregulation has been reported following COVID-19 disease. Prolonged T cell activation is associated with initial disease severity and may be implicated mechanistically in the onset of long-covid symptoms. Here we assess the role of extracellular vesicles (EV) in regulating T cell function over several weeks post COVID-19 infection. We find both the cellular origin and protein content of EV was altered in COVID-19 convalescent individuals compared to healthy donors, with alterations linked to initial disease severity. We demonstrate that convalescent donor-derived EV can alter the function and metabolic rewiring of both CD4 and CD8 T cells. Of note, EV following mild, but not severe disease, show distinctly immunesuppressive properties, reducing T cell effector cytokine production and glucose metabolism. Mechanistically our data indicate the involvement of EV-surface ICAM-1 in facilitating EV - T cell interaction. Taken together, our data demonstrate that circulatory EV are phenotypically and functionally altered several weeks following acute infection, suggesting a role for EV as long-term immune modulators.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:51:11.884.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Howden
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-16 07:07:15	ID requested
1	2023-06-28 05:15:09	announced
⏵ 2	2024-10-22 05:51:12	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: COVID-19, exosomes, Extracellular Vesicles

Anna Schurich
contact affiliation	Department of Infectious Diseases, School of Immunology and Microbial Sciences, King’s College London, UK
contact email	anna.schurich@kcl.ac.uk
lab head
Andrew Howden
contact affiliation	University of Dundee
contact email	a.howden@dundee.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD043058

PRIDE project URI

• PRIDE
  1. PXD043058
     1. Label: PRIDE project
     2. Name: Extracellular Vesicles in COVID-19 convalescence can regulate T cell metabolism and function