PXD043041

PXD043041 is an original dataset announced via ProteomeXchange.

Title	TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis
Description	Proximity labeling (PL) was recently developed to detect protein–protein interactions (PPIs) and members of subcellular multiprotein structures in living cells. Proximity labeling is conducted by fusing an engineered enzyme with catalytic activity, such as biotin ligase, to a protein of interest (bait protein) to biotinylate adjacent proteins. The biotinylated protein can be purified by strep-tavidin beads, and identified by mass spectrometry (MS). TurboID is an engineered biotin ligase with high catalytic efficiency, which is used for proximity labeling. Although TurboID-based proximity labeling technology has been successfully established in mammals, its application in plant systems is limited. Here, we report the usage of TurboID for proximity labeling of FIP37, a core member of m6A methyltransferase complex, to identify FIP37 interacting proteins in Ara-bidopsis thaliana. By analyzing the MS data, we found 214 proteins biotinylated by GFP-TurboID-FIP37 fusion, including five components of m6A methyltransferase complex that have been previously confirmed. Therefore, the identified proteins may include potential proteins directly involved in the m6A pathway or functionally related to m6A-coupled mRNA processing due to spatial proximity. Moreover, we demonstrated the feasibility of proximity labeling tech-nology in plant epitranscriptomics studies, thereby expanding the application of this technology to more subjects of plant research.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_09:05:12.643.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD043041
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	li xiao fang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-15 20:33:26	ID requested
⏵ 1	2024-01-26 09:05:13	announced

10.6019/PXD043041;

10.1002/pld3.555;

Li X, Wei Y, Fei Q, Fu G, Gan Y, Shi C, . Plant Direct, 7(12):e555(2023) [pubmed]

submitter keyword: proximity labeling (PL)

TurboID

protein–protein interactions (PPIs)

biotin

m6A

FIP37

Chuanlin Shi
contact affiliation	Chinese Academy of Agricultural Science
contact email	shichuanlin@caas.cn
lab head
li xiao fang
contact affiliation	Chinese Academy of Agricultural Science
contact email	free8849@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD043041

PRIDE project URI

• PRIDE
  1. PXD043041
     1. Label: PRIDE project
     2. Name: TurboID-mediated proximity labeling for screening interacting proteins of FIP37 in Arabidopsis