PXD043038-1
PXD043038 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PIAS3/TGF-β/TXNIP axis promotes ferroptosis in HCC |
| Description | Ferroptosis has been suggested to have a potential role in cancer therapy as an iron-dependent programmed cell death distinguished from other forms. Hepatocellular carcinoma (HCC) remains a great threat with high lethality and limited therapies. Induction of ferroptosis has emerged as a novel and promising therapeutic strategy for HCC. Here, we identify PIAS3 as a driver of ferroptosis in HCC through TMT-based quantitative proteomics and ferroptosis-related functional assays. Mechanistically, TXNIP is confirmed as a downstream of PIAS3 in promoting ferroptotic cell death based on RNA-seq analysis. The knockdown of TXNIP degrades ferroptotic susceptibility caused by PIAS3-overexpression, whereas the transfection-forced re-expression of TXNIP restores sensitivity to ferroptosis in PIAS3-downregulated cells. In the meantime, PIAS3 interacts with SMAD2/3 to activate TGF-β signaling, which leads to increased TXNIP expression. Our study uncovers the critical role of PIAS3 in ferroptosis and a novel actionable axis-PIAS3/TGF-β/TXNIP that could govern ferroptotic sensitivity, paving the path for targeting ferroptosis as an efficient approach in HCC therapies. |
| HostingRepository | iProX |
| AnnounceDate | 2023-06-15 |
| AnnouncementXML | Submission_2023-06-15_18:50:12.903.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Jingde Chen |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-15 18:49:53 | ID requested | |
| ⏵ 1 | 2023-06-15 18:50:13 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: PIAS3, Ferroptosis, HCC, TGF-β signaling, TXNIP |
Contact List
| Jingde Chen | |
|---|---|
| contact affiliation | Shanghai East Hospital, Tongji University School of Medicine |
| contact email | 1600092@tongji.edu.cn |
| lab head | |
| Jingde Chen | |
| contact affiliation | Shanghai East Hospital, Tongji University School of Medicine |
| contact email | 1600092@tongji.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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