PXD043033-1

PXD043033 is an original dataset announced via ProteomeXchange.

Title	Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria
Description	Cerebral malaria (CM), the most lethal complication of Plasmodium falciparum severe malaria (SM), claims the life of 15 to 25% of admitted children despite treatment. P. falciparum infects and multiplies in human erythrocytes, contributing to anemia, parasite sequestration, and inflammation in the host. In this study, an unbiased proteomic assessment of infected erythrocytes and plasma samples from 24 Beninese children was performed to decipher the complex mechanisms underlying the pathophysiology of CM and the corresponding host-parasite interactions. A significant down-regulation of proteins from the ubiquitin-proteasome pathway and an up-regulation of the erythroid precursor marker transferrin receptor protein 1 (TFRC) was associated with infected erythrocytes from CM patients. Further functional analysis showed dysregulated iron metabolism and ferroptosis pathways associated with CM. At the plasma level, the samples clustered according to clinical presentation. Importantly, increased levels of the 20S proteasome components were associated with SM. Targeted quantification assays confirmed these findings on a larger confirmation cohort (n=274). These findings suggest that parasites causing CM preferentially infect reticulocytes or erythroid precursors and alter their maturation. Importantly, the host plasma proteome serves as a specific signature of SM and presents a remarkable opportunity for developing innovative diagnostic and prognostic biomarkers.
HostingRepository	PRIDE
AnnounceDate	2024-02-02
AnnouncementXML	Submission_2024-02-01_16:37:17.243.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Virginie Salnot
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-15 15:13:31	ID requested
⏵ 1	2024-02-01 16:37:17	announced
2	2024-10-22 06:28:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Severe malaria, Plasma, Plasmodium falciparum, LC-MS/MS

Gwladys I.
contact affiliation	Université Paris Cité, IRD, UMR261, MERIT, F-75006 Paris, France
contact email	gwladys.bertin@ird.fr
lab head
Virginie Salnot
contact affiliation	3p5-proteom'ic plateform
contact email	virginie.salnot@parisdescartes.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD043033
     1. Label: PRIDE project
     2. Name: Infected erythrocytes and plasma proteomics reveal a specific protein signature of severe malaria