PXD043023-1

PXD043023 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	derailed protein turnover in the aging mammalian brain
Description	Efficient protein turnover is essential for cellular homeostasis and organ function. Loss of proteostasis is a hallmark of aging, which culminates as a severe reduction in protein turnover rates. To investigate changes in protein turnover dynamics as a function of age, we performed continuous in vivo metabolic stable isotope labeling in mice along the aging continuum. First, we discovered that the brain proteome uniquely experiences dynamic global turnover fluctuations during aging compared to heart and liver tissue. Second, in the brain proteome, global protein turnover trends across aging displayed sex-specific differences that were tightly tied to their cellular compartments. Next, parallel analyses of the insoluble proteome revealed that distinct cellular compartments experience hampered turnover, in part due to misfolding. Finally, we discovered that age-associated fluctuations in the activity of the ubiquitin proteasome system were linked to the turnover of the catalytic core subunits. Taken together, our study provides a proteome-wide atlas of protein turnover across the aging continuum and highlights a link between the turnover of individual proteasome subunits and the age-associated decline in proteosome activity.
HostingRepository	MassIVE
AnnounceDate	2023-06-15
AnnouncementXML	Submission_2023-06-15_19:12:30.741.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeffrey N. Savas
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	unknown modification
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-15 07:31:27	ID requested
⏵ 1	2023-06-15 19:12:31	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Aging

submitter keyword: Aging

Contact List

Jeffrey N. Savas, PhD
contact affiliation	Department of Neurology Northwestern University
contact email	jeffrey.savas@northwestern.edu
lab head
Jeffrey N. Savas
contact affiliation	Northwestern University
contact email	jeffrey.savas@northwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000092174/