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PXD042976-1

PXD042976 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Plasmodium falciparum CCCH zinc finger protein MD3 regulates male gametocytogenesis through its interaction with RNA-binding proteins
DescriptionMalaria transmission to mosquitoes is dependent on the formation of gametocytes. When fully matured, gametocytes are able to transform into gametes in the mosquito's midgut, a process accompanied with their egress from the enveloping erythrocyte. Gametocyte maturation and gametogenesis require a well-coordinated gene expression programme, involving a wide spectrum of regulatory proteins, ranging from histone modifiers to transcription factors to RNA-binding proteins. Here, we investigated the role of the CCCH-zinc finger protein MD3, in P. falciparum gametocytogenesis. MD3 was originally identified by us as an epigenetically regulated protein of immature gametocytes and recently shown to be involved in male development in a barcode-based screen in P. berghei. In P. falciparum, MD3 is mainly present in the cytoplasm of immature male gametocytes. Parasites deficient of MD3 are impaired in gametocyte maturation and male gametocyte exflagellation. BioID analysis in combination with co-immunoprecipitation assays unveiled an interaction network of MD3 with RNA-binding proteins like PABP1 and ALBA3, with translational initiators, regulators and repressors like elF4G, Puf1, NOT1 and CITH, and with other zinc finger proteins involved in gametocytogenesis, including ZNF4, MD1 and GD1. We conclude that MD3 is part of a regulator complex crucial for post-transcriptional fine-tuning of male gametocytogenesis.
HostingRepositoryjPOST
AnnounceDate2023-12-12
AnnouncementXMLSubmission_2023-12-12_09:23:46.119.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterUte Distler
SpeciesList scientific name: cellular organisms; NCBI TaxID: 131567;
ModificationListS-carboxamidomethyl-L-cysteine; alpha-amino acetylated residue; L-methionine sulfoxide
Instrumentinstrument
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-14 03:15:33ID requested
12023-12-12 09:23:46announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Plasmodium falciparum, interactome, mass spectrometry, proximity-labeling
Contact List
Gabriele Pradel
lab head
Ute Distler
contact affiliationUniversity Medical Center Mainz
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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