PXD042965-1

PXD042965 is an original dataset announced via ProteomeXchange.

Title	BAG5, a co-chaperone protein, regulates the assembly of HTCA in sperm
Description	Acephalic spermatozoa syndrome is severe teratozoospermia, displaying the separation of the sperm head and tail. In 2015, we first reported that genetic ablation of SPATA6 results in the separation of sperm head and tail. Here, we focus on a co-chaperone protein BAG5 that interacts with SPATA6 and expresses in steps 9-16 spermatids. The deficiency of BAG5 in male mice causes the abnormal assembly of the head-tail coupling apparatus (HTCA), leading to acephalic spermatozoa and male infertility. In vivo and in vitro experiments demonstrated that HTCA formation-related proteins (SPATA6), cargo transport-related myosin proteins (MYO5A and MYL6) and dynein proteins (DYNLT1, DCTN1 and DNAL1) were misfolded after loss of BAG5. Further, BAG5 interacts with HSPA8 to regulate its affinity with SPATA6, myosin proteins and dynein proteins. Therefore, we speculate that BAG5 regulates the assembly of the head-tail coupling apparatus (HTCA) by activating the protein-folding function of HSPA8.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:25:33.048.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	世明 甘
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 21:01:12	ID requested
⏵ 1	2023-11-14 09:25:33	announced
2	2024-10-22 06:14:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: acephalic sperm,BAG5, flagellum, infertility, protein-folding

Shiming Gan
contact affiliation	Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
contact email	Shiminggan@hust.edu.cn
lab head
世明 甘
contact affiliation	tongji
contact email	1206682912@qq.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042965
     1. Label: PRIDE project
     2. Name: BAG5, a co-chaperone protein, regulates the assembly of HTCA in sperm