PXD042947-2
PXD042947 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome |
| Description | Membrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ~1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a “liquid biopsy,” while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry in unfractionated plasma. We have developed a one-step enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma. The Mag-Net method is robust, reproducible, inexpensive, and requires <100 μL plasma input. Coupled to a quantitative data-independent mass spectrometry analytical strategy, we demonstrate that we can routinely collect results for >37,000 peptides from >4,000 plasma proteins with high precision. We demonstrate excellent quantitative accuracy and analytical reproducibility of the protocol. |
| HostingRepository | PanoramaPublic |
| AnnounceDate | 2025-07-02 |
| AnnouncementXML | Submission_2025-07-02_20:15:39.673.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Michael MacCoss |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | Carbamidomethyl |
| Instrument | Orbitrap Eclipse |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-13 09:40:48 | ID requested | |
| 1 | 2023-06-13 15:35:38 | announced | |
| ⏵ 2 | 2025-07-02 20:15:40 | announced | : Added PubMed ID |
Publication List
Keyword List
| submitter keyword: extracellular vesicles, DIA mass spectrometry, plasma proteomics, quantitative proteomics, neurodegenerative disease |
Contact List
| Michael MacCoss | |
|---|---|
| contact affiliation | University of Washington |
| contact email | maccoss@uw.edu |
| lab head | |
| Michael MacCoss | |
| contact affiliation | University of Washington |
| contact email | maccoss@uw.edu |
| dataset submitter | |
Full Dataset Link List
| Panorama Public dataset URI |
to receive all new ProteomeXchange dataset release announcements!
