PXD042947

PXD042947 is an original dataset announced via ProteomeXchange.

Title	Mag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome
Description	Membrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ~1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a “liquid biopsy,” while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry in unfractionated plasma. We have developed a one-step enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma. The Mag-Net method is robust, reproducible, inexpensive, and requires <100 μL plasma input. Coupled to a quantitative data-independent mass spectrometry analytical strategy, we demonstrate that we can routinely collect results for >37,000 peptides from >4,000 plasma proteins with high precision. We demonstrate excellent quantitative accuracy and analytical reproducibility of the protocol.
HostingRepository	PanoramaPublic
AnnounceDate	2025-07-02
AnnouncementXML	Submission_2025-07-02_20:15:39.673.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Michael MacCoss
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-13 09:40:48	ID requested
1	2023-06-13 15:35:38	announced
⏵ 2	2025-07-02 20:15:40	announced	: Added PubMed ID

Wu CC, Tsantilas KA, Park J, Plubell D, Sanders JA, Naicker P, Govender I, Buthelezi S, Stoychev S, Jordaan J, Merrihew G, Huang E, Parker ED, Riffle M, Hoofnagle AN, Noble WS, Poston KL, Montine TJ, MacCoss MJ, Enrichment of extracellular vesicles using Mag-Net for the analysis of the plasma proteome. Nat Commun, 16(1):5447(2025) [pubmed]

submitter keyword: extracellular vesicles, DIA mass spectrometry, plasma proteomics, quantitative proteomics, neurodegenerative disease

Michael MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
lab head
Michael MacCoss
contact affiliation	University of Washington
contact email	maccoss@uw.edu
dataset submitter

Panorama Public dataset URI