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PXD042947 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMag-Net: Rapid enrichment of membrane-bound particles enables high coverage quantitative analysis of the plasma proteome
DescriptionMembrane-bound particles in plasma are composed of exosomes, microvesicles, and apoptotic bodies and represent ~1-2% of the total protein composition. Proteomic interrogation of this subset of plasma proteins augments the representation of tissue-specific proteins, representing a “liquid biopsy,” while enabling the detection of proteins that would otherwise be beyond the dynamic range of liquid chromatography-tandem mass spectrometry in unfractionated plasma. We have developed a one-step enrichment strategy (Mag-Net) using hyper-porous strong-anion exchange magnetic microparticles to sieve membrane-bound particles from plasma. The Mag-Net method is robust, reproducible, inexpensive, and requires <100 μL plasma input. Coupled to a quantitative data-independent mass spectrometry analytical strategy, we demonstrate that we can routinely collect results for >37,000 peptides from >4,000 plasma proteins with high precision. We demonstrate excellent quantitative accuracy and analytical reproducibility of the protocol.
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichael MacCoss
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
InstrumentOrbitrap Eclipse
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-13 09:40:48ID requested
12023-06-13 15:35:38announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: extracellular vesicles, DIA mass spectrometry, plasma proteomics, quantitative proteomics, neurodegenerative disease
Contact List
Michael MacCoss
contact affiliationUniversity of Washington
contact emailmaccoss@uw.edu
lab head
Michael MacCoss
contact affiliationUniversity of Washington
contact emailmaccoss@uw.edu
dataset submitter
Full Dataset Link List
Panorama Public dataset URI