PXD042940 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteomic analysis of extracellular vesicle released by MCF7 cells in the absence of the tetraspanins CD9, CD81 or CD63 |
| Description | The tetraspanins CD9, CD81, and CD63 are among the most widely used markers of Extracellular Vesicles (EVs) and are also used to isolate the vesicles they decorate. If their function in EVs is largely unknown, they have been suggested to play a role in the sorting of protein cargos into EVs or to regulate the uptake of EVs by acceptor cells. Using different tetraspanin-deficient MCF7 breast cancer cell lines, we have shown that the absence of any of these 3 tetraspanins had a minimal impact on their protein composition, as analyzed by label-free quantitative mass spectrometry. However, the EVs released by cells deficient in both CD9 and CD81 showed a decrease level of CD9P-1/EWI-F and EWI-2 (encoded by the genes PTGFRN and IGSF8 respectively), which are two well-characterized CD9 and CD81 molecular partners. |
| HostingRepository | PRIDE |
| AnnounceDate | 2024-10-22 |
| AnnouncementXML | Submission_2024-10-22_05:59:40.257.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD042940 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Cédric Pionneau |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | timsTOF Pro |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2023-06-13 03:47:16 | ID requested | |
| 1 | 2023-08-03 02:09:53 | announced | |
| 2 | 2023-11-14 06:58:28 | announced | 2023-11-14: Updated project metadata. |
| ⏵ 3 | 2024-10-22 05:59:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
| submitter keyword: tetraspanins, exosomes, MCF7, PTGFRN, mass spectrometry, CD9,Extracellular vesicles, CD81, IgSF8, CD63, proteome, timsTOF Pro |
Contact List
| Eric RUBINSTEIN |
|---|
| contact affiliation | Sorbonne Université, Inserm, CNRS, Centre d'Immunologie et des Maladies Infectieuses, CIMI-Paris, F-75013 Paris, France |
| contact email | eric.rubinstein@inserm.fr |
| lab head | |
| Cédric Pionneau |
|---|
| contact affiliation | Plateforme P3S UMS-37 PASS – Sorbonne Universite |
| contact email | cedric.pionneau@sorbonne-universite.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/08/PXD042940 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042940
- Label: PRIDE project
- Name: Proteomic analysis of extracellular vesicle released by MCF7 cells in the absence of the tetraspanins CD9, CD81 or CD63
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