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PXD042922-1

PXD042922 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleTurboID-mediated proximal labeling of Drosophila caspase Drice in adult brains
DescriptionV5-TurboID was knock-in tagged in Drosophila caspase Drice which is expressed in adult brains. Flies were fed with 100 µM biotin for protein biotinylation in two biological replicates. Biotinylated protein-containing lysate extracted from adult brains was subjected to FG-NeutrAvidin beads purification followed by on-beads trypsin digestion. Digested peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
HostingRepositoryjPOST
AnnounceDate2024-02-06
AnnouncementXMLSubmission_2024-02-06_05:15:07.162.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNatsuki Shinoda
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListL-cysteine methyl disulfide; alpha-amino acetylated residue; L-methionine sulfoxide; ubiquitination signature dipeptidyl lysine
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-12 19:53:00ID requested
12024-02-06 05:15:07announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Drice, Caspase, Drosophila, TurboID, LFQ
Contact List
Masayuki Miura
lab head
Natsuki Shinoda
contact affiliationThe University of Tokyo
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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