PXD042871-1
PXD042871 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Validity of the cell-extracted proteome as a substrate pool for exploring phosphorylation motifs of kinases |
| Description | Three representative protein kinases with different substrate preference including ERK1 (pro-directed), CK2 (acidophilic), and PKA (basophilic) and the substrate pool proteome from three different cell lines including MCF7 (human mammary carcinoma), HeLa (human cervical carcinoma), Jurkat (human acute T-cell leukemia) were used to evaluate phosphorylation sequence motifs. Specifically, recombinant kinases were added to the cell-extracted proteome, phosphorylated in vitro, trypsin digested, and the phosphopeptides were enriched for nanoLC/MS/MS analysis to identify their phosphorylation sites on a large scale. By analyzing the obtained phosphorylation sites and their surrounding sequences, phosphorylation motifs were extracted for each kinase-substrate proteome pair. The results showed that each kinase exhibited identical phosphorylation motifs, independent of the substrate proteome. Furthermore, the motifs were also consistent with those from completely randomized peptide libraries. These results indicate that cell-extracted proteomes can provide kinase phosphorylation motifs with sufficient accuracy, even though their sequences are not completely random, supporting the robustness of the phosphorylation motifs from the phosphoproteome analysis of cell extracts as a substrate pool for the kinase of interest. |
| HostingRepository | jPOST |
| AnnounceDate | 2024-06-11 |
| AnnouncementXML | Submission_2024-06-10_08:00:20.321.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Tomoya Niinae |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine; O4'-phospho-L-tyrosine |
| Instrument | instrument |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-11 00:59:10 | ID requested | |
| ⏵ 1 | 2024-06-10 08:00:20 | announced |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: Kinase, Phosphoproteome |
Contact List
| Yasushi Ishihama | |
|---|---|
| lab head | |
| Tomoya Niinae | |
| contact affiliation | Kyoto university |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST002188/ |
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