PXD042781-2

PXD042781 is an original dataset announced via ProteomeXchange.

Title	HEK cells expressing miRFP680-P2A-GFP, emiRFP670-P2A-GFP, emiRFP703-P2A-GFP, miRFP713-P2A-GFP and miRFP720-P2A-GFP
Description	HEK cells transiently transfected with pAAV-CAG-NIR-FPs-P2A-GFP plasmids were processed using Mini MS Sample Prep kit according to the manufacture protocol. Extracted peptides were analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) in data-independent acquisition (DIA) mode. We reported the protein identity and relative concentration of NIR FPs and GFPs for each analyzed sample as well as post-translational modifications if any are identified.
HostingRepository	iProX
AnnounceDate	2023-06-07
AnnouncementXML	Submission_2025-06-12_21:16:07.840.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Hanbin Zhang
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 21:16:56	ID requested
1	2023-06-06 21:17:04	announced
⏵ 2	2025-06-12 21:16:08	announced	2025-06-13: Add Publication.

Zhang H, Papadaki S, Sun X, Wang X, Drobizhev M, Yao L, Rehbock M, K, ö, ster RW, Wu L, Namikawa K, Piatkevich KD, Quantitative assessment of near-infrared fluorescent proteins. Nat Methods, 20(10):1605-1616(2023) [pubmed]

submitter keyword: fluorescent proteins

Kiryl Piatkevich
contact affiliation	Westlake University
contact email	kiryl.piatkevich@westlake.edu.cn
lab head
Hanbin Zhang
contact affiliation	Westlake University
contact email	zhanghanbin@westlake.edu.cn
dataset submitter

iProX dataset URI