PXD042773 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mechanism of orphan subunit recognition during assembly quality control_BioMass@LMB |
Description | Cells contain numerous abundant molecular machines assembled from multiple subunits. Imbalances in subunit production and failed assembly generate orphan subunits that are eliminated by poorly defined pathways. Here, we determined how orphan subunits of the cytosolic chaperonin CCT are recognized. Several unassembled CCT subunits recruited the E3 ubiquitin ligase HERC2 using ZNRD2 as an adaptor. Both factors were necessary for orphan CCT subunit degradation in cells, sufficient for CCT subunit ubiquitination with purified factors, and necessary for optimal cell fitness. Domain mapping and structure prediction defined the molecular features of a minimal HERC2-ZNRD2-CCT module. The structural model, whose key elements were validated in cells using point mutants, shows why ZNRD2 selectively recognizes multiple orphaned CCT subunits without engaging assembled CCT. Our findings reveal how failures during CCT assembly are monitored and provide a paradigm for the molecular recognition of orphan subunits, the largest source of quality control substrates in cells. |
HostingRepository | PRIDE |
AnnounceDate | 2023-07-26 |
AnnouncementXML | Submission_2023-07-26_07:33:23.967.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | CatarinaFranco |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-06-06 12:40:48 | ID requested | |
⏵ 1 | 2023-07-26 07:33:24 | announced | |
2 | 2023-11-14 06:44:26 | announced | 2023-11-14: Updated project metadata. |
Publication List
Keyword List
submitter keyword: chaperonin CCT, subunit composition |
Contact List
Ramanujan S.Hegde |
contact affiliation | MRC Laboratory of Molecular Biology, Cambridge, United Kingdom |
contact email | rhegde@mrc-lmb.cam.ac.uk |
lab head | |
CatarinaFranco |
contact affiliation | MRC Laboratory of Molecular Biology |
contact email | cffranco@mrc-lmb.cam.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042773
- Label: PRIDE project
- Name: Mechanism of orphan subunit recognition during assembly quality control_BioMass@LMB