PXD042763-1

PXD042763 is an original dataset announced via ProteomeXchange.

Title	O-GlcNAcylation promotes Palbociclib resistance in breast cancer by regulating MITF nuclear translocation
Description	Cyclin-dependent kinases 4 and 6 (CDK4/6) are essential drivers of the cell cycle and are also critical for the initiation and progression of diverse malignancies. Pharmacological inhibitors targeting CDK4/6 have demonstrated significant activity against various tumor types such as breast cancer. However, resistance to CDK4/6 inhibitors (CDK4/6i) (such as palbociclib) remains an immense obstacle in clinical and the underlying mechanisms have not been fully understood. Using quantitative high-throughput combinational screen (qHTCS) and genomic sequencing, we report that the Microphthalmia-associated transcription factor (MITF), was significantly elevated in palbociclib-resistance cells. Inhibition of MITF can enhance the therapeutic efficacy of Palbociclib and surmount Palbociclib resistance both in vitro and in vivo. Then we found that MITF is interacting with OGT in Palbociclib-resistance cells, and we also identified that Ser49 is the main O-GlcNAc site of MITF. O-GlcNAc of MITF is critical to mediated Palbociclib-resistance in cells.
HostingRepository	PRIDE
AnnounceDate	2024-05-30
AnnouncementXML	Submission_2024-05-30_07:23:17.646.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	YI ZHANG
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LCQ Classic

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 08:06:58	ID requested
⏵ 1	2024-05-30 07:23:18	announced
2	2024-10-22 06:43:21	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: O-GlcNAcylation, MITF, Palbociclib

Wenge Zhu
contact affiliation	George Washington University
contact email	wz6812@gwu.edu
lab head
YI ZHANG
contact affiliation	The George Washington University
contact email	scholarzy@gwu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/05/PXD042763

PRIDE project URI

• PRIDE
  1. PXD042763
     1. Label: PRIDE project
     2. Name: O-GlcNAcylation promotes Palbociclib resistance in breast cancer by regulating MITF nuclear translocation