PXD042761-2

PXD042761 is an original dataset announced via ProteomeXchange.

Title	LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance
Description	Fully grown oocytes remain transcriptionally quiescent, yet many maternal mRNAs are synthesized and retained in growing oocytes. We now know that maternal mRNAs are stored in a structure called the mitochondria associated ribonucleoprotein domain (MARDO). But the components and functions of MARDO remain elusive. Here, we found that LSM14B knockout prevents the proper storage and timely clearance of mRNAs (including Cyclin B1, Btg4, and other mRNAs that are translationally activated during meiotic maturation), specifically by disrupting MARDO assembly during oocyte growth and meiotic maturation. With decreased levels of storage and clearance, the LSM14B knockout oocytes failed to enter meiosis II, ultimately resulting in female infertility. Our results demonstrate the function of LSM14B in MARDO assembly, couple the MARDO with mRNA clearance and oocyte meiotic maturation
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:12:28.319.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Y.L. Wan
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-06 07:10:51	ID requested
1	2023-10-17 02:54:23	announced
⏵ 2	2023-11-14 07:12:28	announced	2023-11-14: Updated project metadata.
⏵ 2	2023-11-14 07:12:28	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: LSM14B

oocyte meiotic maturation

mRNA storage

Hongbing Liu
contact affiliation	Center for Reproductive Medicine, Shandong University
contact email	hongbin_sduivf@aliyun.com
lab head
Y.L. Wan
contact affiliation	Center for Reproductive Medicine, Shandong University
contact email	wyl9208@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042761

PRIDE project URI

• PRIDE
  1. PXD042761
     1. Label: PRIDE project
     2. Name: LSM14B is essential for oocyte meiotic maturation by regulating maternal mRNA storage and clearance