PXD042744-2
PXD042744 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | PPTC7 integrates homeostatic and physiological signals to regulate mitophagy and mitochondrial mass |
| Description | Here we report that knockout of PPTC7, a mitochondrial matrix protein, hyperactivates BNIP3/NIX-mediated mitophagy in vitro and in vivo. Biochemically, the PPTC7 precursor is trapped by BNIP3 and NIX to mitochondrial outer-membrane, where PPTC7 scaffolds the assembly of the substrate-PPTC7- SCFFBXL4 holocomplex to degrade BNIP3 and NIX, forming a homeostatic regulatory loop. PPTC7 possesses an unusually weak mitochondrial targeting sequence to facilitate its outer-membrane retention and mitophagy control. Upon starvation, PPTC7 is transcriptionally upregulated in mouse liver to repress mitophagy, which critically maintains hepatic mitochondrial mass, bioenergetics and gluconeogenesis. Collectively, PPTC7 functions as a mitophagy sensor that integrates homeostatic and physiological signals to dynamically control BNIP3 and NIX degradation, thereby maintaining proper mitochondrial mass and cellular homeostasis. |
| HostingRepository | iProX |
| AnnounceDate | 2023-06-05 |
| AnnouncementXML | Submission_2024-05-29_21:37:57.082.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Yuqiu Sun |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | acetylated residue; methionine oxidation with neutral loss of 64 Da |
| Instrument | LTQ Orbitrap Velos; Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2023-06-05 20:28:42 | ID requested | |
| 1 | 2023-06-05 20:29:07 | announced | |
| ⏵ 2 | 2024-05-29 21:37:57 | announced | 2024-05-30: Update information. |
Publication List
| Sun Y, Cao Y, Wan H, Memetimin A, Cao Y, Li L, Wu C, Wang M, Chen S, Li Q, Ma Y, Dong M, Jiang H, A mitophagy sensor PPTC7 controls BNIP3 and NIX degradation to regulate mitochondrial mass. Mol Cell, 84(2):327-344.e9(2024) [pubmed] |
Keyword List
| submitter keyword: PPTC7, FBXL4, mitophagy receptors BNIP3 and NIX, ubiquitination, mitochondial mass, metabolism |
Contact List
| Hui Jiang | |
|---|---|
| contact affiliation | National Institute of Biological Sciences, Beijing |
| contact email | jianghui@nibs.ac.cn |
| lab head | |
| Yuqiu Sun | |
| contact affiliation | National Institute of Biological Sciences, Beijing |
| contact email | sunyuqiu@nibs.ac.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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