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PXD042744-2

PXD042744 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePPTC7 integrates homeostatic and physiological signals to regulate mitophagy and mitochondrial mass
DescriptionHere we report that knockout of PPTC7, a mitochondrial matrix protein, hyperactivates BNIP3/NIX-mediated mitophagy in vitro and in vivo. Biochemically, the PPTC7 precursor is trapped by BNIP3 and NIX to mitochondrial outer-membrane, where PPTC7 scaffolds the assembly of the substrate-PPTC7- SCFFBXL4 holocomplex to degrade BNIP3 and NIX, forming a homeostatic regulatory loop. PPTC7 possesses an unusually weak mitochondrial targeting sequence to facilitate its outer-membrane retention and mitophagy control. Upon starvation, PPTC7 is transcriptionally upregulated in mouse liver to repress mitophagy, which critically maintains hepatic mitochondrial mass, bioenergetics and gluconeogenesis. Collectively, PPTC7 functions as a mitophagy sensor that integrates homeostatic and physiological signals to dynamically control BNIP3 and NIX degradation, thereby maintaining proper mitochondrial mass and cellular homeostasis.
HostingRepositoryiProX
AnnounceDate2023-06-05
AnnouncementXMLSubmission_2024-05-29_21:37:57.082.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterYuqiu Sun
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListacetylated residue; methionine oxidation with neutral loss of 64 Da
InstrumentLTQ Orbitrap Velos; Orbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-06-05 20:28:42ID requested
12023-06-05 20:29:07announced
22024-05-29 21:37:57announced2024-05-30: Update information.
Publication List
Sun Y, Cao Y, Wan H, Memetimin A, Cao Y, Li L, Wu C, Wang M, Chen S, Li Q, Ma Y, Dong M, Jiang H, A mitophagy sensor PPTC7 controls BNIP3 and NIX degradation to regulate mitochondrial mass. Mol Cell, 84(2):327-344.e9(2024) [pubmed]
Keyword List
submitter keyword: PPTC7, FBXL4, mitophagy receptors BNIP3 and NIX, ubiquitination, mitochondial mass, metabolism
Contact List
Hui Jiang
contact affiliationNational Institute of Biological Sciences, Beijing
contact emailjianghui@nibs.ac.cn
lab head
Yuqiu Sun
contact affiliationNational Institute of Biological Sciences, Beijing
contact emailsunyuqiu@nibs.ac.cn
dataset submitter
Full Dataset Link List
iProX dataset URI