PXD042713-1

PXD042713 is an original dataset announced via ProteomeXchange.

Title	Proteome characterization of XPC-deficient melanocytes generated by CRISPR-Cas9 technology reveals alteration in the expression of several hundred of proteins
Description	Xeroderma pigmentosum (XP) is a rare autosomal recessive disorder owing to the defects in one of the factors implicated in nucleotide excision repair (NER) system. The effect of NER deficiency on melanocyte biology has not received much attention up to now from a mechanistic standpoint, due to the lack of an appropriate model. Therefore, we decided to generate XPC-deficient primary melanocytes by CRISPR-Cas9 technology, herein after called ©-XPC melanocytes. To this end, we designed two gRNA for exon 9 close to the most common XPC mutation (i.e. c.1643_1644delTG). These gRNA were cloned into lentiviral vectors containing the Cas9 nuclease and puromycin selection cassettes. Following transduction with lentivirus, melanocytes sustainably expressing Cas-9 and scramble gRNA (©-Ctrl) or gRNA targeting XPC gene (©-XPC) were subjected to PCR analysis, Sanger sequencing and western blotting. Following validation of our model, ©-Ctrl and ©-XPC melanocytes were subjected to a quantitative label-free differential proteomic analysis.
HostingRepository	PRIDE
AnnounceDate	2024-12-17
AnnouncementXML	Submission_2024-12-17_02:14:06.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dupuy Jean-William
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-04 11:48:03	ID requested
⏵ 1	2024-12-17 02:14:07	announced

Cario M, Scalia J, Mahfouf W, Muzotte E, Michaud V, Plaisant C, Dupuy JW, Douki T, Morice-Picard F, Rambert J, Perrier E, Trompezinski S, Rezvani HR, Proteome characterization of XPC-deficient melanocytes generated by CRISPR-Cas9 technology reveals alteration in the expression of several hundred proteins. J Dermatol Sci, 114(2):79-82(2024) [pubmed]

10.1016/j.jdermsci.2024.03.006;

submitter keyword: immune system, xeroderma pigmentosum, melanocyte,Proteomic

Hamid Reza Rezvani
contact affiliation	Université de Bordeaux, INSERM, UMR1312, Bordeaux Institute of Oncology, Bordeaux, France.
contact email	hamid-reza.rezvani@u-bordeaux.fr
lab head
Dupuy Jean-William
contact affiliation	OncoProt plateform, UAR TBMCore CNRS 3427 INSERM US005 University of Bordeaux
contact email	jean-william.dupuy@u-bordeaux.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042713
     1. Label: PRIDE project
     2. Name: Proteome characterization of XPC-deficient melanocytes generated by CRISPR-Cas9 technology reveals alteration in the expression of several hundred of proteins