PXD042643-2

PXD042643 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the mucus of the photosynthetic sea slug Elysia crispata
Description	Elysia crispata is a tropical sea slug Sacoglossa is a superorder of marine sea slugs, of which a few speciesthat can retain intracellular functional chloroplasts from their its algae prey, a mechanism termed kleptoplasty. Elysia crispata is a tropical species of Sacoglossa that can feed through this mechanism on and acquire chloroplasts from a variety of macroalgae. Thisese sea slugs, as other gastropods, produce mucus, a viscous secretion with multiple functions, such as lubrication, protection, and locomotion. This study presents the first comprehensive analysis of the mucus proteome of the sea slug E. crispata using gel electrophoresis and HPLC-MS/MS. We identified 306 proteins in the mucus secretions of this animal, despite the limited entries for E. crispata in the Uniprot database. The reproducibility of the mucus sampling technique was evaluated revealing no significant differences in protein abundance across samples. The functional annotation of the mucus proteome using Gene Ontology identified proteins involved in different functions such as hydrolase activity (molecular function), carbohydrate-derived metabolic processes (biological processes) and cytoskeletal organization (cell component). Moreover, a high proportion of proteins with enzymatic activity in the mucus of E. crispata suggests potential biotechnological applications including antimicrobial and antitumor activities. Putative antimicrobial properties are reinforced by the high abundance of hydrolases. This study also identified proteins common in mucus samples from various species, supporting a common mechanism of mucus in protecting cells and tissues while facilitating animal movement. This study highlights the need for further research to fully understand the roles of these proteins in mucus, their potential impact on animal physiology, and the influence of genetics, and environmental factors, including the type of mucus, on protein composition and relative abundance.
HostingRepository	PRIDE
AnnounceDate	2024-01-23
AnnouncementXML	Submission_2024-01-23_07:34:29.623.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042643
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Diana Lopes
SpeciesList	scientific name: Elysia crispata; NCBI TaxID: 231223;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-02 03:07:37	ID requested
1	2024-01-22 02:51:03	announced
⏵ 2	2024-01-23 07:34:30	announced	2024-01-23: Updated project metadata.
3	2024-10-22 06:24:25	announced	2024-10-22: Updated project metadata.

10.1016/J.JPROT.2024.105087;

10.6019/PXD042643;

submitter keyword: elysia crispata, sea sug, mucus, proteome

Pedro Miguel
contact affiliation	Mass Spectrometry Centre, LAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, Portugal
contact email	p.domingues@ua.pt
lab head
Diana Lopes
contact affiliation	University of Aveiro
contact email	lopes.diana@ua.pt
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042643
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the mucus of the photosynthetic sea slug Elysia crispata