PXD042627-1

PXD042627 is an original dataset announced via ProteomeXchange.

Title	Metaproteomic insights into the elemental stoichiometry of a changing Southern Ocean
Description	We conducted two iron-temperature bioassay incubation experiments during the austral summer of 2018-2019 in the Weddell Sea, Southern Ocean. The first bioassay (BA1) was initiated on December 28th, 2018, approximately 560 km away from the ice edge at 65°S 0.038°E. The second bioassay (BA2) was initiated on January 9th, 2019, approximately 70 km from the ice edge at 70.15°S 11.02°W (Figure 1A). For both bioassays, we collected sea water at 20 m depth using a custom-built ultraclean CTD sampling system – “Titan” (De Baar et al. 2008). We chose this depth to minimize light shock when the microbial community was brought on deck, and to prevent trace metal contamination from the ship. After recovery, Titan was moved into a dedicated, temperature-controlled, trace-metal-clean (TMC) laboratory container where the sea water was used for initial (T0) sampling, and to fill triplicate, 20 L, TMC cubitainers for each iron-temperature treatment. The treatment matrix including four iron-temperature conditions: low iron – low temperature, high iron – low temperature, low iron – high temperature, high iron – high temperature. We did not add any iron to the low iron treatments. We supplemented the high iron treatments with a naturally occurring but rare isotope of 57Fe, which allowed us to differentiate between the added iron and iron already present in the water (mainly 56Fe). For this, we made a 40 µM 57FeCl3 stock solution in 0.1 M trace-metal grade HCl, then added 1 mL of this stock to each of the high iron cubitainers. This resulted in an initial dissolved 57Fe concentration of 2 nM in the high iron treatments. We then sealed the cubitainers and placed them inside temperature-controlled deck incubators where the low temperature treatments were incubated under in situ temperature, and the high temperature treatments were incubated under in situ + 2 °C (Table S1).
HostingRepository	PRIDE
AnnounceDate	2026-06-24
AnnouncementXML	Submission_2026-06-24_10:00:28.044.xml
DigitalObjectIdentifier	https://doi.org/10.6019/PXD042627
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Loay Jabre
SpeciesList	scientific name: marine plankton environmental sample; NCBI TaxID: NEWT:632957;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-06-01 10:22:20	ID requested
⏵ 1	2026-06-24 10:00:28	announced

10.1101/2025.10.03.680396;

10.6019/PXD042627;

submitter keyword: Southern Ocean, trace metals, diatoms, temperature,Phytoplankton, iron, Phaeocystis, metaproteomics, elemental stoichiometry

Erin M. Bertrand
contact affiliation	Department of Biology, Dalhousie University, Halifax, Nova Scotia, Canada
contact email	erin.bertrand@dal.ca
lab head
Loay Jabre
contact affiliation	Dalhousie University
contact email	loay.jabre@dal.ca
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042627
     1. Label: PRIDE project
     2. Name: Metaproteomic insights into the elemental stoichiometry of a changing Southern Ocean