PXD042587-2

PXD042587 is an original dataset announced via ProteomeXchange.

Title	In situ observation of chaperonin function in protein folding
Description	The GroEL/GroES chaperonin mediates protein folding in bacteria in an ATP-dependent process. Studies in vitro show that the GroEL double-ring and the lid-shaped GroES transiently encapsulate unfolded protein for folding unimpaired by aggregation. To clarify critical aspects of this mechanism, we used cryo-electron tomography in situ to visualize and quantify functional GroEL:ES complexes in their intact cellular environment. Mass spectrometry was used to estimate stoichiometries of GroEL, GroES, cellular ribosome content and substrates.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:47:37.554.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Roman Körner
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-31 10:58:17	ID requested
1	2024-06-27 01:34:10	announced
⏵ 2	2024-10-22 06:47:37	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Chaperonin, ATPase, CHAPERONE, heat shock, Folding cage, proteostasis

F. Ulrich Hartl
contact affiliation	Max-Planck Institute of Biochemistry, Department of Cellular Biochemistry
contact email	uhartl@biochem.mpg.de
lab head
Roman Körner
contact affiliation	Max-Planck Institute of Biochemistry, Department of Cellular Biochemistry
contact email	rkoerner@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/06/PXD042587

PRIDE project URI

• PRIDE
  1. PXD042587
     1. Label: PRIDE project
     2. Name: In situ observation of chaperonin function in protein folding