PXD042577 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Trazodone is neuroprotective via rescue of dysregulated synaptic and mitochondrial translatomes in neurodegeneration |
Description | We aime to discover changes which occur at the hippocampal nascent proteome prion to the neurodegeneration characteristic of prion disease and how this prion signature difer from that of proven neuroprotective drug trazodone hydrochloride. To label the nacent proteome we used non-canonical aminoacid tagging (NCAT) labelling all cell types within the hippocampus with L-azidohomoalanine (AHA) as well as azinorleucine (ANL) to label specifically Camk2a or GFAP neurons or astrocytes respectivly. We gave methionine surrogates mixed in drinking water and soggy mash for 1 week after which animals were sacrificed and both hippocampi extracted. Affinity purification of this samples showed a restoration of Mitochondrial and syaptic proteins decrease with prion disease which 2 week trazdoone treatement restored. |
HostingRepository | PRIDE |
AnnounceDate | 2024-05-24 |
AnnouncementXML | Submission_2024-05-24_03:00:24.883.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Hector Albert Gasco |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2023-05-31 07:43:38 | ID requested | |
⏵ 1 | 2024-05-24 03:00:25 | announced | |
Publication List
10.1093/brain/awad313; |
Albert-Gasco H, Smith HL, Alvarez-Castelao B, Swinden D, Halliday M, Janaki-Raman S, Butcher AJ, Mallucci GR, Trazodone rescues dysregulated synaptic and mitochondrial nascent proteomes in prion neurodegeneration. Brain, 147(2):649-664(2024) [pubmed] |
Keyword List
submitter keyword: synapse, UPR/ISR, trazodone,neurodegeneration, translational repression, mutochondria, translatome |
Contact List
Giovanna Mallucci |
contact affiliation | Cambridge institute of Science, Altos Labs, Cambridge, UK |
contact email | gmallucci@altoslabs.com |
lab head | |
Hector Albert Gasco |
contact affiliation | Altos labs |
contact email | hector.hv2@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD042577
- Label: PRIDE project
- Name: Trazodone is neuroprotective via rescue of dysregulated synaptic and mitochondrial translatomes in neurodegeneration