PXD042543-2

PXD042543 is an original dataset announced via ProteomeXchange.

Title	Screening of exosome-derived proteins and their potential as biomarkers to diagnostic and prognostic for pancreatic cancer
Description	This study aimed to investigate the proteomic landscape of plasma exosomes from patients diagnosed with PDAC and IPMN, compared to healthy controls (CT), in order to identify potential biomarkers with prognostic and diagnostic values. Additionally, the study aimed to understand the functional role of the exosome's proteome in pancreatic neoplasia. We isolated the plasma exosomes and analyzed its proteome by nano electrospray tandem mass spectrometry (nanoLC-MS/MS), and performed downstream statistical and bioinformatics analysis, using the Perseus (v. 6.2.2), MSigDB (v. 2023.1), STRING (v. 11.5), and NCG (v. 7.1) databases. A total of 319 differentially expressed proteins (DEPs) were identified among the exosome samples, but our analyses focused on the 135 proteins that were expressed in at least 70% of each group's samples to ensure their representative proteomes. Comparing PDAC and IPMN exosome proteomes to controls, we identified 24 and 33 differentially expressed proteins, respectively. Some of the most upregulated proteins in PDAC exosomes were CPN1, IGHV2-26, ITIH3, and CLU, while some of the most downregulated were C4BPB, APOB, CFH, and C1QB. In IPMN exosomes, KLKB1, LBP, CFB, and SERPINA1 were the most upregulated proteins while C5, APOD, C3, and C1QA were downregulated. In general, the protein-protein networks and enrichment analyses revealed interesting interaction clusters and pathways related to cancer development, such as the immune system, complement cascade, clotting-related and vesicle-mediated processes, regulation of insulin-like growth factor transport, platelet activation signaling, and G-protein signaling. Our analyses also highlighted candidate cancer drivers, including the upregulated IGLL, LBP, SERPINA1, SERPINA4, SERPING1, and the downregulated APOB, and C3, which were identified in these pathways and may have potential roles in pancreatic tumorigenesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:02:32.780.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michel Batista
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-30 07:10:35	ID requested
1	2023-09-04 07:11:04	announced
⏵ 2	2024-10-22 06:02:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Mass Spectrometry,Pancreatic cancer, Exosome, Proteomics, Biomarkers

Mateus Nobrega Aoki
contact affiliation	Laboratory for Applied Science and Technology in Health, Carlos Chagas Institute, Oswaldo Cruz Foundation (Fiocruz),Curitiba, Brazil
contact email	mateus.aoki@fiocruz.br
lab head
Michel Batista
contact affiliation	Fiocruz
contact email	batista.mic@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042543
     1. Label: PRIDE project
     2. Name: Screening of exosome-derived proteins and their potential as biomarkers to diagnostic and prognostic for pancreatic cancer