PXD042541-1

PXD042541 is an original dataset announced via ProteomeXchange.

Title	Multi-Tiered Chemical Proteomic Maps of Tryptoline Acrylamide-Protein Interactions in Cancer Cells
Description	Covalent chemistry is a versatile approach for expanding the ligandability of the human proteome. Activity-based protein profiling (ABPP) can infer the specific residues modified by electrophilic compounds through competition with broadly reactive probes. Nonetheless, the extent to which such residue-directed ABPP platforms are capable of fully mapping the protein targets of electrophilic compounds in human cells remains unclear. Here, we introduce a complementary approach that directly identifies proteins showing stereoselective reactivity with focused libraries of stereochemically-defined, alkynylated electrophilic compounds. Integration of protein- and cysteine-directed ABPP data from compound-treated human cancer cells revealed generally well-correlated target maps and highlighted specific features, such as protein size and the proteotypicity of cysteine-containing peptides, that help to explain gaps in each ABPP platform. The integrated ABPP strategy furnished stereoselective, high-engagement covalent ligands for > 300 structurally and functionally diverse human proteins, including compounds that modulate enzymes by targeting isotype-restricted and non-catalytic cysteines.
HostingRepository	PRIDE
AnnounceDate	2024-06-08
AnnouncementXML	Submission_2024-06-07_18:18:19.502.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	EVERT NJOMEN
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse; Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-30 06:28:14	ID requested
⏵ 1	2024-06-07 18:18:19	announced

Dataset with its publication pending

submitter keyword: covalent chemistry, Chemoproteomics, Stereoprobes, protein-directed ABPP

Benjamin F
contact affiliation	Department of Chemistry, The Scripps Research Institute, La Jolla, CA, USA
contact email	cravatt@scripps.edu
lab head
EVERT NJOMEN
contact affiliation	Scripps Research Institute
contact email	enjomen@scripps.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042541
     1. Label: PRIDE project
     2. Name: Multi-Tiered Chemical Proteomic Maps of Tryptoline Acrylamide-Protein Interactions in Cancer Cells