PXD042540-1

PXD042540 is an original dataset announced via ProteomeXchange.

Title	Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans
Description	The methyltransferase-like protein 13 (METTL13) methylates the eukaryotic elongation factor 1 alpha (eEF1A) on two locations: the N-terminal amino group and lysine 55. The absence of this methylation leads to reduced protein synthesis and cell proliferation in human cancer cells. Previous studies showed that METTL13 is dispensable in non-transformed cells, making it potentially interesting for cancer therapy. However, METTL13 has not been examined yet in whole animals. Here, we used the nematode Caenorhabditis elegans as a simple model to assess the functions of METTL13. Using methyltransferase assays and mass spectrometry, we show that the C. elegans METTL13 (METL-13) methylates eEF1A (EEF-1A) in the same way as the human protein. Crucially, the cancer-promoting role of METL-13 is also conserved and depends on the methylation of EEF-1A, like in human cells. At the same time, METL-13 appears dispensable for animal growth, development, and stress responses. This makes C. elegans a convenient whole-animal model for studying METL13-dependent carcinogenesis without the complications of interfering with essential wild-type functions.
HostingRepository	PRIDE
AnnounceDate	2023-07-03
AnnouncementXML	Submission_2023-07-03_03:02:37.801.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042540
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	BerndThiede
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	monomethylated residue; S-carboxamidoethyl-L-cysteine; monohydroxylated residue
Instrument	timsTOF Pro; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-30 06:28:07	ID requested
⏵ 1	2023-07-03 03:02:38	announced
2	2023-11-14 09:01:58	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:51:42	announced	2024-10-22: Updated project metadata.

10.6019/PXD042540;

Engelfriet ML, Ma, ł, ecki JM, Forsberg AF, Falnes P Ø, Ciosk R, Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans. PLoS One, 18(6):e0287558(2023) [pubmed]

submitter keyword: METTL13, methylation, C. elegans, eEF1A, protein synthesis

Jedrzej M.Malecki
contact affiliation	Department of Biosciences University of Oslo
contact email	j.m.malecki@ibv.uio.no
lab head
BerndThiede
contact affiliation	University of Oslo
contact email	bernd.thiede@ibv.uio.no
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042540
     1. Label: PRIDE project
     2. Name: Characterization of the biochemical activity and tumor-promoting role of the dual protein methyltransferase METL-13/METTL13 in Caenorhabditis elegans