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PXD042513-1

PXD042513 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCombined transcriptomics and proteomics analysis unveiled the impact of vitamin C in modulating specific mitochondrial protein abundance in the mouse liver
Description: In the present study, we used the Gulo-/- female and male mice model which depends entirely on ascorbate derived from the diet. We tested different concentrations of ascorbate ranging from 0 to 0.4% (w/v) ascorbate, added into drinking water, until the age of four months. Importantly, the levels of ascorbate found in the liver of Gulo-/- mice reflect the amounts of ascorbate provided in drinking water. We performed label-free Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) global quantitative proteomic profiling to identify and quantify proteins in whole liver extracts from our different ascorbate treated cohorts of Gulo-/- females and males. We compared the proteomic profiles to the transcriptomic profiles of the same treated animals. Although several proteins of the mitochondrial complex III significantly correlated with vitamin C concentrations, their corresponding transcripts did not correlate with vitamin C in both females and males. Such observations were confirmed by western blot and quantitative RT-PCR analyses, respectively. Our findings suggest that transcriptome profiling alone provides an incomplete picture of molecular changes associated with vitamin C deficiency in the liver of mice and examination of changes in protein abundances is essential to unveil variations that are not transcriptionally regulated.
HostingRepositoryMassIVE
AnnounceDate2024-04-30
AnnouncementXMLSubmission_2024-04-30_07:53:10.532.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMichel Lebel
SpeciesList scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList4-hydroxy-L-proline
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-26 08:28:14ID requested
12024-04-30 07:53:11announced
Publication List
no publication
Keyword List
submitter keyword: Vitamin C, gulonolactone oxidase, mass spectrometry, mitochondrial complex III, transcription, liver, mouse
Contact List
Michel Lebel
contact affiliationCentre Hospitalier Universite Laval
contact emailmichel.lebel@crchudequebec.ulaval.ca
lab head
Michel Lebel
contact affiliationCHudeQuebe
contact emailmichel.lebel@crchudequebec.ulaval.ca
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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