PXD042504-1

PXD042504 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 -1 PRF RNA Pull Down and SFL Pull Down
Description	To identify the protein partners of SARS-CoV-2 -1 PRF RNA, we performed RNA pull-down assays using streptavidin magnetic beads. To obtain more accurate and reliable data, tRSA sequence-tagged -1 PRF and -1 FSE RNA probes were both used to capture binding proteins in our screen. As a random sequence control for nonspecificity, the equivalent proteins extracted from H1299 cells were incubated with tRSA-labeled -1 PRF and -1 FSE RNA probes. A high-throughput technology, LC/MS-MS(MS), was applied to identify the candidate interacting proteins of SARS-CoV-2 -1 PRF RNA.
HostingRepository	iProX
AnnounceDate	2023-05-25
AnnouncementXML	Submission_2023-05-25_19:43:26.949.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tanxiu Chen
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-25 19:43:05	ID requested
⏵ 1	2023-05-25 19:43:27	announced

Dataset with its publication pending

submitter keyword: SARS-CoV-2, -1 PRF RNA, SFL, mass spectrometry

Xiaozhong Peng
contact affiliation	Chinese Academy of Medical Sciences, and Peking Union Medical College
contact email	pengxiaozhong@pumc.edu.cn
lab head
Tanxiu Chen
contact affiliation	Chinese Academy of Medical Sciences, and Peking Union Medical College
contact email	chentanxiu@163.com
dataset submitter

iProX dataset URI