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PXD042504

PXD042504 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSARS-CoV-2 -1 PRF RNA Pull Down and SFL Pull Down
DescriptionTo identify the protein partners of SARS-CoV-2 -1 PRF RNA, we performed RNA pull-down assays using streptavidin magnetic beads. To obtain more accurate and reliable data, tRSA sequence-tagged -1 PRF and -1 FSE RNA probes were both used to capture binding proteins in our screen. As a random sequence control for nonspecificity, the equivalent proteins extracted from H1299 cells were incubated with tRSA-labeled -1 PRF and -1 FSE RNA probes. A high-throughput technology, LC/MS-MS(MS), was applied to identify the candidate interacting proteins of SARS-CoV-2 -1 PRF RNA.
HostingRepositoryiProX
AnnounceDate2023-05-25
AnnouncementXMLSubmission_2023-05-25_19:43:26.949.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterTanxiu Chen
SpeciesList scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02023-05-25 19:43:05ID requested
12023-05-25 19:43:27announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: SARS-CoV-2, -1 PRF RNA, SFL, mass spectrometry
Contact List
Xiaozhong Peng
contact affiliationChinese Academy of Medical Sciences, and Peking Union Medical College
contact emailpengxiaozhong@pumc.edu.cn
lab head
Tanxiu Chen
contact affiliationChinese Academy of Medical Sciences, and Peking Union Medical College
contact emailchentanxiu@163.com
dataset submitter
Full Dataset Link List
iProX dataset URI