PXD042502-1

PXD042502 is an original dataset announced via ProteomeXchange.

Title	Parkinson’s VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B
Description	The D620N VPS35 mutation disrupts retrograde endosome to Golgi retromer Trafficking. Thus causes lysosome dysfunction, enhances LRRK2 kinase activity and leads to Parkinson’s disease. We employed a LysoTag immunoprecipitation approach to assess how this impacts lysosomes using quantitative proteomics. The VPS35[D620N] mutation alters the expression of over 350 lysosomal proteins, and induces recruitment of LRRK2 phosphorylated Rab proteins to the lysosome, as well as the phosphoRab effector protein RILPL1.
HostingRepository	PRIDE
AnnounceDate	2023-11-28
AnnouncementXML	Submission_2023-11-28_09:21:08.606.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuko Pui Yiu Lam
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-25 10:06:45	ID requested
⏵ 1	2023-11-28 09:21:09	announced

Dataset with its publication pending

submitter keyword: Parinson's disease

Lysosomal proteins

LRRK2

RILPL1

TMEM55B

Dario R. Alessi
contact affiliation	University of Dundee
contact email	d.r.alessi@dundee.ac.uk
lab head
Yuko Pui Yiu Lam
contact affiliation	University of Dundee
contact email	PLam001@dundee.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD042502
     1. Label: PRIDE project
     2. Name: Parkinson’s VPS35[D620N] retromer lysosomal dysfunction induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55B