PXD042496-1

PXD042496 is an original dataset announced via ProteomeXchange.

Title	Amoxicillin treatment of pneumococcal pneumonia impacts bone marrow neutrophil maturation and function.
Description	Pneumonia caused by Streptococcus pneumoniae is a leading cause of death worldwide, and bacterial resistance to antimicrobial drugs has become a major issue. A growing body of evidence indicates that the successful treatment of bacterial infections results from synergy between antibiotic-mediated direct antibacterial activity and the host’s immune defenses. However, the mechanisms underlying the protective immune responses induced by amoxicillin (a β-lactam antibiotic used as the first-line treatment of S. pneumoniae infections) have not been characterized. A better understanding of amoxicillin’s effects on host-pathogen interactions might facilitate the development of other treatment options. Given the crucial role of neutrophils in the control of S. pneumoniae infections, we decided to investigate amoxicillin’s impact on neutrophil development in a mouse model of pneumococcal superinfection. Although a single therapeutic dose of amoxicillin prevented local and systemic inflammatory responses, it did not impair the emergency granulopoiesis triggered in the bone marrow by S. pneumoniae. Importantly, treatment of pneumonia with amoxicillin was associated with a greater mature neutrophil count in the bone marrow; these neutrophils had specific transcriptomic and proteomic profiles. Furthermore, amoxicillin-conditioned, mature neutrophils in the bone marrow had a less activated phenotype and might be rapidly mobilized in peripheral tissues in response to systemic inflammation. Thus, by revealing a novel effect of amoxicillin on the development and functions of bone marrow neutrophils during Streptococcus pneumoniae pneumonia, our findings provide new insights into the impact of amoxicillin treatment on host immune responses.
HostingRepository	PRIDE
AnnounceDate	2023-10-09
AnnouncementXML	Submission_2023-10-09_12:48:00.997.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD042496
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	SALIOUJean-Michel
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Streptococcus pneumoniae; NCBI TaxID: NCBITaxon:1313;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2023-05-25 06:34:15	ID requested
⏵ 1	2023-10-09 12:48:01	announced

10.6019/PXD042496;

submitter keyword: Neutrophils

Bone marrow

Granulopoiesis

Amoxicillin

Streptococcus pneumoniae

Jean-michelSaliou
contact affiliation	Univ. Lille, CNRS, Inserm, CHU Lille, Institut Pasteur de Lille, US41 – UAR 2014 – PLBS, F-59000 Lille, France
contact email	jean-michel.saliou@pasteur-lille.fr
lab head
SALIOUJean-Michel
contact affiliation	Institut Pasteur de Lille
contact email	jean-michel.saliou@pasteur-lille.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD042496

PRIDE project URI

• PRIDE
  1. PXD042496
     1. Label: PRIDE project
     2. Name: Amoxicillin treatment of pneumococcal pneumonia impacts bone marrow neutrophil maturation and function.